Detailed information NTG0862609

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Q7416_RS21810
• Genome accession: NZ_CP131479
• Coordinates: 4418548..4419348 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain KP_NORM_BLD_111598
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4413548..4424348

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7416_RS21775 (Q7416_21720)	gspH	4413701..4414216 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
Q7416_RS21780 (Q7416_21725)	gspI	4414213..4414578 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
Q7416_RS21785 (Q7416_21730)	gspJ	4414575..4415171 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
Q7416_RS21790 (Q7416_21735)	gspK	4415168..4416148 (+)	981	WP_077266377.1	type II secretion system minor pseudopilin GspK	-
Q7416_RS21795 (Q7416_21740)	gspL	4416141..4417337 (+)	1197	WP_023339600.1	type II secretion system protein GspL	-
Q7416_RS21800 (Q7416_21745)	-	4417330..4417815 (+)	486	WP_012968921.1	type II secretion system protein M	-
Q7416_RS21805 (Q7416_21750)	-	4417805..4418551 (+)	747	WP_012968922.1	type II secretion system protein N	-
Q7416_RS21810 (Q7416_21755)	pilD	4418548..4419348 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
Q7416_RS21815 (Q7416_21760)	hrpB	4419395..4421824 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
Q7416_RS21820 (Q7416_21765)	thpR	4421897..4422433 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
Q7416_RS21825 (Q7416_21770)	sfsA	4422433..4423149 (+)	717	WP_064144629.1	DNA/RNA nuclease SfsA	-
Q7416_RS21830 (Q7416_21775)	dksA	4423312..4423767 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=862609 Q7416_RS21810 WP_008807384.1 4418548..4419348(+) (pilD) [Klebsiella variicola strain KP_NORM_BLD_111598]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=862609 Q7416_RS21810 WP_008807384.1 4418548..4419348(+) (pilD) [Klebsiella variicola strain KP_NORM_BLD_111598]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATTACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425