Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Q5H80_RS13090 Genome accession   NZ_CP130748
Coordinates   2941324..2942349 (-) Length   341 a.a.
NCBI ID   WP_304565363.1    Uniprot ID   -
Organism   Vibrio sp. SNU_ST1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2936324..2947349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5H80_RS13065 (Q5H80_13065) aroK 2937150..2937668 (-) 519 WP_009848907.1 shikimate kinase AroK -
  Q5H80_RS13070 (Q5H80_13070) pilQ 2937845..2939539 (-) 1695 WP_369809718.1 type IV pilus secretin PilQ Machinery gene
  Q5H80_RS13075 (Q5H80_13075) pilP 2939599..2940117 (-) 519 WP_304569420.1 pilus assembly protein PilP Machinery gene
  Q5H80_RS13080 (Q5H80_13080) pilO 2940104..2940733 (-) 630 WP_304565359.1 type 4a pilus biogenesis protein PilO Machinery gene
  Q5H80_RS13085 (Q5H80_13085) pilN 2940726..2941340 (-) 615 WP_304565361.1 PilN domain-containing protein Machinery gene
  Q5H80_RS13090 (Q5H80_13090) pilM 2941324..2942349 (-) 1026 WP_304565363.1 type IV pilus assembly protein PilM Machinery gene
  Q5H80_RS13095 (Q5H80_13095) - 2942501..2945059 (+) 2559 WP_304565365.1 penicillin-binding protein 1A -
  Q5H80_RS13100 (Q5H80_13100) oxyR 2945171..2946064 (-) 894 WP_009848900.1 DNA-binding transcriptional regulator OxyR -
  Q5H80_RS13105 (Q5H80_13105) - 2946286..2947014 (+) 729 WP_010435300.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38134.56 Da        Isoelectric Point: 6.1302

>NTDB_id=861316 Q5H80_RS13090 WP_304565363.1 2941324..2942349(-) (pilM) [Vibrio sp. SNU_ST1]
MGPSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRAGALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPEKGNWLLVDVGVDQTSLGIIPQDSAPFYKDIAFGTQDLRSTDAPDDIESVFGSAEDTH
QFIVHLIEKLKRQLQLYSSVNAQQPISGIWLMGEGASTPMVIEELERHFQLSCEPLNPLSLFEDKVAKRHRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=861316 Q5H80_RS13090 WP_304565363.1 2941324..2942349(-) (pilM) [Vibrio sp. SNU_ST1]
ATGGGTCCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTCCTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCTATTTCGGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAATTAGAAGACAGAGAGAAAGAGTTTTCTATTTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGATCGACAA
GTAGCTATCAGGTATATGCGACTCGTAAGGAAGTTGTAGACAGTCGAGCGGGGGCTTTGACTAAGGCTGGCTTTAAACCT
GTTGTGGTGGATACGCAGGCGCATGGGCTTCTCAATATTTGGCAGTTGGCTTCACGCCTTTATCCTGAAAAAGGTAACTG
GTTGCTGGTGGACGTGGGAGTTGACCAAACGTCGTTAGGCATTATTCCACAAGATTCGGCACCGTTTTACAAAGATATCG
CCTTTGGTACCCAAGACCTTCGTAGCACTGATGCCCCGGATGATATTGAGAGTGTGTTCGGTAGCGCTGAGGATACCCAT
CAATTTATTGTTCATTTAATTGAAAAGCTCAAGCGTCAATTGCAGCTCTATTCATCAGTGAATGCGCAACAACCTATCTC
TGGGATCTGGCTGATGGGCGAGGGCGCTAGCACGCCGATGGTGATTGAAGAGCTAGAACGTCATTTTCAGCTGAGCTGTG
AGCCATTGAATCCCTTGTCTCTGTTTGAGGACAAGGTAGCGAAGAGACACCGACTACCTATGGACTGGCAACACTTTGGT
ATTGCGGCGGGTATGGCGATGAGTGGGCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.545

100

0.545
  pilM Vibrio campbellii strain DS40M4

49.267

100

0.493