Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ86_RS19295 Genome accession   NZ_CP147084
Coordinates   3914242..3914919 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain KTa005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3909242..3919919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ86_RS19260 (WAZ86_19255) gspG 3909722..3910159 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  WAZ86_RS19265 (WAZ86_19260) gspH 3910167..3910676 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  WAZ86_RS19270 (WAZ86_19265) gspI 3910673..3911050 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WAZ86_RS19275 (WAZ86_19270) gspJ 3911043..3911630 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WAZ86_RS19280 (WAZ86_19275) gspK 3911623..3912606 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WAZ86_RS19285 (WAZ86_19280) gspL 3912621..3913784 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  WAZ86_RS19290 (WAZ86_19285) gspM 3913781..3914242 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  WAZ86_RS19295 (WAZ86_19290) pilD 3914242..3914919 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  WAZ86_RS19300 (WAZ86_19295) bfr 3914948..3915424 (-) 477 WP_000675504.1 bacterioferritin -
  WAZ86_RS19305 (WAZ86_19300) bfd 3915496..3915690 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WAZ86_RS19310 (WAZ86_19305) chiA 3915859..3918552 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=858757 WAZ86_RS19295 WP_000178161.1 3914242..3914919(+) (pilD) [Escherichia coli strain KTa005]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=858757 WAZ86_RS19295 WP_000178161.1 3914242..3914919(+) (pilD) [Escherichia coli strain KTa005]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378