Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ54_RS06520 Genome accession   NZ_CP147056
Coordinates   1224354..1225163 (-) Length   269 a.a.
NCBI ID   WP_000545349.1    Uniprot ID   -
Organism   Escherichia coli strain 1002W03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1219354..1230163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ54_RS06505 (WAZ54_06510) gspD 1220811..1222871 (-) 2061 WP_000498830.1 type II secretion system secretin GspD -
  WAZ54_RS06510 (WAZ54_06515) gspC 1222901..1223860 (-) 960 WP_001229917.1 type II secretion system protein GspC -
  WAZ54_RS06515 (WAZ54_06520) gspS2 1223878..1224288 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  WAZ54_RS06520 (WAZ54_06525) pilD 1224354..1225163 (-) 810 WP_000545349.1 prepilin peptidase PppA Machinery gene
  WAZ54_RS06525 (WAZ54_06530) sslE 1225293..1229858 (-) 4566 WP_001034546.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29471.08 Da        Isoelectric Point: 8.3829

>NTDB_id=858229 WAZ54_RS06520 WP_000545349.1 1224354..1225163(-) (pilD) [Escherichia coli strain 1002W03]
MHFDVFQQYPAVMPILATVGGLVIGSFLNVVIWRYPIMLRQQMAEFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=858229 WAZ54_RS06520 WP_000545349.1 1224354..1225163(-) (pilD) [Escherichia coli strain 1002W03]
ATGCATTTTGATGTTTTTCAGCAATATCCAGCGGTGATGCCCATCCTGGCAACCGTTGGAGGATTGGTCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTACGC
AGTCAAAAATAAGCCTGGCGCTGCCACGCTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGCTCACGCTACAAGACGCAGTTACCGGCGTACTGGTGGGATTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGGCGTTATCACTGCCCAACGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

41.328

100

0.416
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368