Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   Q2U85_RS22140 Genome accession   NZ_CP130335
Coordinates   4302759..4303802 (-) Length   347 a.a.
NCBI ID   WP_088068503.1    Uniprot ID   -
Organism   Bacillus cereus strain DW444     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4297759..4308802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2U85_RS22095 (Q2U85_22095) - 4298192..4298392 (-) 201 WP_000106083.1 YqzE family protein -
  Q2U85_RS22100 (Q2U85_22100) aroK 4298431..4298928 (-) 498 WP_153587805.1 shikimate kinase AroK -
  Q2U85_RS22105 (Q2U85_22105) - 4299044..4299694 (-) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  Q2U85_RS22110 (Q2U85_22110) comGG 4299869..4300240 (-) 372 WP_088065610.1 competence type IV pilus minor pilin ComGG -
  Q2U85_RS22115 (Q2U85_22115) comGF 4300237..4300707 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  Q2U85_RS22120 (Q2U85_22120) - 4300677..4300979 (-) 303 WP_153587853.1 type II secretion system protein -
  Q2U85_RS22125 (Q2U85_22125) comGD 4300972..4301427 (-) 456 WP_088068508.1 competence type IV pilus minor pilin ComGD -
  Q2U85_RS22130 (Q2U85_22130) comGC 4301424..4301723 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  Q2U85_RS22135 (Q2U85_22135) comGB 4301735..4302766 (-) 1032 WP_088068505.1 competence type IV pilus assembly protein ComGB -
  Q2U85_RS22140 (Q2U85_22140) comGA 4302759..4303802 (-) 1044 WP_088068503.1 competence type IV pilus ATPase ComGA Machinery gene
  Q2U85_RS22145 (Q2U85_22145) - 4304008..4304703 (+) 696 WP_000434124.1 metalloregulator ArsR/SmtB family transcription factor -
  Q2U85_RS22150 (Q2U85_22150) - 4304830..4305072 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  Q2U85_RS22155 (Q2U85_22155) - 4305180..4306574 (+) 1395 WP_001094334.1 L-cystine transporter -
  Q2U85_RS22160 (Q2U85_22160) - 4306654..4306992 (-) 339 WP_228492666.1 hypothetical protein -
  Q2U85_RS22165 (Q2U85_22165) - 4307134..4307349 (-) 216 WP_001008326.1 DUF3912 family protein -
  Q2U85_RS22170 (Q2U85_22170) - 4307602..4307913 (+) 312 WP_001093237.1 hypothetical protein -
  Q2U85_RS22175 (Q2U85_22175) - 4307948..4308436 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39183.56 Da        Isoelectric Point: 8.5415

>NTDB_id=858007 Q2U85_RS22140 WP_088068503.1 4302759..4303802(-) (comGA) [Bacillus cereus strain DW444]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=858007 Q2U85_RS22140 WP_088068503.1 4302759..4303802(-) (comGA) [Bacillus cereus strain DW444]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCAACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369