Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V9Z40_RS02800 Genome accession   NZ_CP146667
Coordinates   575467..576900 (-) Length   477 a.a.
NCBI ID   WP_000809054.1    Uniprot ID   -
Organism   Escherichia coli strain MS 16-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570467..581900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9Z40_RS02765 (V9Z40_02765) elbB 571158..571811 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  V9Z40_RS02770 (V9Z40_02770) mtgA 571808..572536 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V9Z40_RS02775 (V9Z40_02775) yrbL 572533..573165 (-) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  V9Z40_RS02780 (V9Z40_02780) npr 573379..573651 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  V9Z40_RS02785 (V9Z40_02785) rapZ 573648..574502 (-) 855 WP_000243741.1 RNase adapter RapZ -
  V9Z40_RS02790 (V9Z40_02790) ptsN 574548..575039 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V9Z40_RS02795 (V9Z40_02795) hpf 575157..575444 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  V9Z40_RS02800 (V9Z40_02800) rpoN/rpoN1 575467..576900 (-) 1434 WP_000809054.1 RNA polymerase factor sigma-54 Machinery gene
  V9Z40_RS02805 (V9Z40_02805) lptB 576948..577673 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  V9Z40_RS02810 (V9Z40_02810) lptA 577680..578237 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V9Z40_RS02815 (V9Z40_02815) lptC 578206..578781 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  V9Z40_RS02820 (V9Z40_02820) kdsC 578778..579344 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V9Z40_RS02825 (V9Z40_02825) kdsD 579365..580351 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  V9Z40_RS02830 (V9Z40_02830) yrbG 580365..581342 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53966.76 Da        Isoelectric Point: 4.3639

>NTDB_id=856604 V9Z40_RS02800 WP_000809054.1 575467..576900(-) (rpoN/rpoN1) [Escherichia coli strain MS 16-3]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELSSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=856604 V9Z40_RS02800 WP_000809054.1 575467..576900(-) (rpoN/rpoN1) [Escherichia coli strain MS 16-3]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCAAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCATGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAATATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAGCAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463