Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QZL35_RS14345 Genome accession   NZ_CP129940
Coordinates   3125837..3126430 (-) Length   197 a.a.
NCBI ID   WP_064354357.1    Uniprot ID   A0AAW7A2J1
Organism   Vibrio alginolyticus strain I25B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120837..3131430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZL35_RS14325 aroB 3121722..3122822 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  QZL35_RS14330 aroK 3122855..3123373 (-) 519 WP_005381319.1 shikimate kinase AroK -
  QZL35_RS14335 pilQ 3123561..3125306 (-) 1746 WP_315867617.1 type IV pilus secretin PilQ family protein Machinery gene
  QZL35_RS14340 pilP 3125332..3125847 (-) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  QZL35_RS14345 pilO 3125837..3126430 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  QZL35_RS14350 pilN 3126423..3126998 (-) 576 WP_064354358.1 PilN domain-containing protein Machinery gene
  QZL35_RS14355 pilM 3126982..3127998 (-) 1017 WP_064354359.1 type IV pilus biogenesis protein PilM Machinery gene
  QZL35_RS14360 - 3128143..3130704 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0885

>NTDB_id=856583 QZL35_RS14345 WP_064354357.1 3125837..3126430(-) (pilO) [Vibrio alginolyticus strain I25B]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=856583 QZL35_RS14345 WP_064354357.1 3125837..3126430(-) (pilO) [Vibrio alginolyticus strain I25B]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.842

96.447

0.838
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68