Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   KJS76_RS24020 Genome accession   NZ_AP024513
Coordinates   5211955..5213547 (+) Length   530 a.a.
NCBI ID   WP_023090845.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate Pa12     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5206955..5218547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJS76_RS24000 (RVB2_47720) pgeF 5208705..5209433 (-) 729 WP_003119426.1 peptidoglycan editing factor PgeF -
  KJS76_RS24005 (RVB2_47730) rluD 5209430..5210392 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  KJS76_RS24010 (RVB2_47740) - 5210538..5211563 (+) 1026 WP_160501296.1 outer membrane protein assembly factor BamD -
  KJS76_RS24015 (RVB2_47750) - 5211720..5211965 (+) 246 WP_003102591.1 PP0621 family protein -
  KJS76_RS24020 (RVB2_47760) pilS 5211955..5213547 (+) 1593 WP_023090845.1 two-component system sensor histidine kinase PilS Regulator
  KJS76_RS24025 (RVB2_47770) pilR 5213562..5214899 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  KJS76_RS24030 (RVB2_47780) thiO 5214945..5216039 (-) 1095 WP_003119424.1 glycine oxidase ThiO -
  KJS76_RS24035 (RVB2_47790) - 5216174..5216683 (+) 510 WP_003161431.1 Tfp pilus assembly protein FimT/FimU -
  KJS76_RS24040 (RVB2_47800) - 5216789..5217295 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  KJS76_RS24045 (RVB2_47810) pilV 5217286..5217843 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58971.13 Da        Isoelectric Point: 6.6885

>NTDB_id=85618 KJS76_RS24020 WP_023090845.1 5211955..5213547(+) (pilS) [Pseudomonas aeruginosa isolate Pa12]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSDLEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=85618 KJS76_RS24020 WP_023090845.1 5211955..5213547(+) (pilS) [Pseudomonas aeruginosa isolate Pa12]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGACCTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTACTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCACTAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTGGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGAAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.434

100

0.994

Multiple sequence alignment