Detailed information NTG0855364

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QYQ59_RS04325
• Genome accession: NZ_CP129755
• Coordinates: 932636..933436 (-)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain 2020C01-081
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 927636..938436

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QYQ59_RS04305 (QYQ59_04305)	dksA	928217..928672 (-)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-
QYQ59_RS04310 (QYQ59_04310)	sfsA	928835..929551 (-)	717	WP_049157674.1	DNA/RNA nuclease SfsA	-
QYQ59_RS04315 (QYQ59_04315)	thpR	929551..930087 (-)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
QYQ59_RS04320 (QYQ59_04320)	hrpB	930160..932589 (+)	2430	WP_046620841.1	ATP-dependent helicase HrpB	-
QYQ59_RS04325 (QYQ59_04325)	pilD	932636..933436 (-)	801	WP_008807384.1	A24 family peptidase	Machinery gene
QYQ59_RS04330 (QYQ59_04330)	-	933433..934179 (-)	747	WP_086070387.1	type II secretion system protein N	-
QYQ59_RS04335 (QYQ59_04335)	-	934169..934654 (-)	486	WP_044613514.1	type II secretion system protein M	-
QYQ59_RS04340 (QYQ59_04340)	gspL	934647..935843 (-)	1197	WP_044613515.1	type II secretion system protein GspL	-
QYQ59_RS04345 (QYQ59_04345)	gspK	935836..936816 (-)	981	WP_077257383.1	type II secretion system minor pseudopilin GspK	-
QYQ59_RS04350 (QYQ59_04350)	gspJ	936813..937409 (-)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
QYQ59_RS04355 (QYQ59_04355)	gspI	937406..937771 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
QYQ59_RS04360 (QYQ59_04360)	gspH	937768..938283 (-)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=855364 QYQ59_RS04325 WP_008807384.1 932636..933436(-) (pilD) [Klebsiella variicola strain 2020C01-081]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=855364 QYQ59_RS04325 WP_008807384.1 932636..933436(-) (pilD) [Klebsiella variicola strain 2020C01-081]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425