Detailed information NTG0853595

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QY486_RS01995
• Genome accession: NZ_CP129496
• Coordinates: 429861..431009 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CW41
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 424861..436009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QY486_RS01970 (QY486_01970)	-	426012..426308 (-)	297	WP_034029486.1	DUF167 domain-containing protein	-
QY486_RS01975 (QY486_01975)	-	426305..426898 (-)	594	WP_003084544.1	YggT family protein	-
QY486_RS01980 (QY486_01980)	proC	426909..427730 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
QY486_RS01985 (QY486_01985)	-	427742..428434 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QY486_RS01990 (QY486_01990)	pilT	428649..429683 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QY486_RS01995 (QY486_01995)	pilU	429861..431009 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QY486_RS02000 (QY486_02000)	czcD	431017..431916 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QY486_RS02005 (QY486_02005)	-	432071..432475 (+)	405	WP_003084560.1	NINE protein	-
QY486_RS02010 (QY486_02010)	-	432718..434091 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
QY486_RS02015 (QY486_02015)	-	434088..435272 (+)	1185	WP_301424066.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=853595 QY486_RS01995 WP_003100959.1 429861..431009(+) (pilU) [Pseudomonas aeruginosa strain CW41]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=853595 QY486_RS01995 WP_003100959.1 429861..431009(+) (pilU) [Pseudomonas aeruginosa strain CW41]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382