Detailed information NTG0085339

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: QUE29_RS16980
• Genome accession: NZ_AP027258
• Coordinates: 3442104..3442763 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain JNE151685
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3437104..3447763

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUE29_RS16955 (JNE151685_32310)	ygiS	3438387..3439994 (-)	1608	WP_097753825.1	ABC transporter substrate-binding protein	-
QUE29_RS16960 (JNE151685_32320)	mqsA	3440127..3440522 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
QUE29_RS16965 (JNE151685_32330)	mqsR	3440524..3440820 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
QUE29_RS16970 (JNE151685_32340)	ygiV	3441025..3441507 (-)	483	WP_000183494.1	GyrI-like domain-containing protein	-
QUE29_RS16975 (JNE151685_32350)	ygiW	3441560..3441952 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
QUE29_RS16980 (JNE151685_32360)	ciaR	3442104..3442763 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
QUE29_RS16985 (JNE151685_32370)	qseC	3442760..3444109 (+)	1350	WP_053889563.1	quorum sensing histidine kinase QseC	-
QUE29_RS16990 (JNE151685_32380)	mdaB	3444219..3444800 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
QUE29_RS16995 (JNE151685_32390)	ygiN	3444831..3445145 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
QUE29_RS17000 (JNE151685_32400)	parE	3445193..3447085 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-
QUE29_RS17005 (JNE151685_32410)	yqiA	3447114..3447695 (-)	582	WP_000105733.1	esterase YqiA	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=85339 QUE29_RS16980 WP_001221502.1 3442104..3442763(+) (ciaR) [Escherichia coli strain JNE151685]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=85339 QUE29_RS16980 WP_001221502.1 3442104..3442763(+) (ciaR) [Escherichia coli strain JNE151685]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365