Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE29_RS16540 Genome accession   NZ_AP027258
Coordinates   3351178..3352158 (-) Length   326 a.a.
NCBI ID   WP_115724171.1    Uniprot ID   -
Organism   Escherichia coli strain JNE151685     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3346178..3357158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE29_RS16510 (JNE151685_31450) yggI 3346833..3347330 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  QUE29_RS16515 (JNE151685_31460) endA 3347425..3348132 (+) 708 WP_000286500.1 deoxyribonuclease I -
  QUE29_RS16520 (JNE151685_31470) rsmE 3348212..3348943 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUE29_RS16525 (JNE151685_31480) gshB 3348956..3349906 (+) 951 WP_000593273.1 glutathione synthase -
  QUE29_RS16530 (JNE151685_31490) yqgE 3350015..3350578 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  QUE29_RS16535 (JNE151685_31500) ruvX 3350578..3350994 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  QUE29_RS16540 (JNE151685_31510) pilT 3351178..3352158 (-) 981 WP_115724171.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE29_RS16545 (JNE151685_31520) yggS 3352176..3352880 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QUE29_RS16550 (JNE151685_31530) yggT 3352898..3353464 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QUE29_RS16555 (JNE151685_31540) yggU 3353461..3353751 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  QUE29_RS16560 (JNE151685_31550) rdgB 3353759..3354352 (+) 594 WP_182533439.1 XTP/dITP diphosphatase -
  QUE29_RS16565 (JNE151685_31560) hemW 3354345..3355481 (+) 1137 WP_000239917.1 radical SAM family heme chaperone HemW -
  QUE29_RS16570 (JNE151685_31570) yggM 3355636..3356643 (-) 1008 WP_000745217.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35979.23 Da        Isoelectric Point: 6.1257

>NTDB_id=85335 QUE29_RS16540 WP_115724171.1 3351178..3352158(-) (pilT) [Escherichia coli strain JNE151685]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAEKQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=85335 QUE29_RS16540 WP_115724171.1 3351178..3352158(-) (pilT) [Escherichia coli strain JNE151685]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAAACAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCTCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Acinetobacter baylyi ADP1

37.273

100

0.377
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377

Multiple sequence alignment