Detailed information NTG0848042

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QUC25_RS02235
• Genome accession: NZ_CP128505
• Coordinates: 469827..470975 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain Fe8-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464827..475975

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUC25_RS02210 (QUC25_02210)	-	465978..466274 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QUC25_RS02215 (QUC25_02215)	-	466271..466864 (-)	594	WP_003084544.1	YggT family protein	-
QUC25_RS02220 (QUC25_02220)	proC	466875..467696 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QUC25_RS02225 (QUC25_02225)	-	467708..468400 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QUC25_RS02230 (QUC25_02230)	pilT	468615..469649 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QUC25_RS02235 (QUC25_02235)	pilU	469827..470975 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
QUC25_RS02240 (QUC25_02240)	czcD	470983..471882 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QUC25_RS02245 (QUC25_02245)	-	472037..472441 (+)	405	WP_003084560.1	NINE protein	-
QUC25_RS02250 (QUC25_02250)	-	472684..474057 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
QUC25_RS02255 (QUC25_02255)	-	474054..475238 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=848042 QUC25_RS02235 WP_003110016.1 469827..470975(+) (pilU) [Pseudomonas aeruginosa strain Fe8-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=848042 QUC25_RS02235 WP_003110016.1 469827..470975(+) (pilU) [Pseudomonas aeruginosa strain Fe8-1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ATTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382