Detailed information NTG0847857

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QTN46_RS13150
• Genome accession: NZ_CP128501
• Coordinates: 2433663..2434277 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain SRCM123386
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2428663..2439277

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QTN46_RS13120 (QTN46_13120)	rpsT	2428963..2429229 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QTN46_RS13125 (QTN46_13125)	holA	2429246..2430287 (-)	1042	Protein_2575	DNA polymerase III subunit delta	-
QTN46_RS13130 (QTN46_13130)	-	2430327..2430481 (-)	155	Protein_2576	hypothetical protein	-
QTN46_RS13135 (QTN46_13135)	-	2430522..2430656 (+)	135	WP_003152870.1	YqzM family protein	-
QTN46_RS13140 (QTN46_13140)	comEC	2430675..2433026 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QTN46_RS13145 (QTN46_13145)	-	2433027..2433596 (-)	570	WP_003152868.1	ComE operon protein 2	-
QTN46_RS13150 (QTN46_13150)	comEA	2433663..2434277 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
QTN46_RS13155 (QTN46_13155)	comER	2434336..2435157 (+)	822	WP_013352943.1	late competence protein ComER	-
QTN46_RS13160 (QTN46_13160)	-	2435226..2435963 (-)	738	WP_013352944.1	class I SAM-dependent methyltransferase	-
QTN46_RS13165 (QTN46_13165)	rsfS	2435960..2436316 (-)	357	WP_003152864.1	ribosome silencing factor	-
QTN46_RS13170 (QTN46_13170)	yqeK	2436334..2436894 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QTN46_RS13175 (QTN46_13175)	-	2436884..2437453 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
QTN46_RS13180 (QTN46_13180)	yhbY	2437464..2437754 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
QTN46_RS13185 (QTN46_13185)	aroE	2437748..2438590 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=847857 QTN46_RS13150 WP_013352942.1 2433663..2434277(-) (comEA) [Bacillus amyloliquefaciens strain SRCM123386]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=847857 QTN46_RS13150 WP_013352942.1 2433663..2434277(-) (comEA) [Bacillus amyloliquefaciens strain SRCM123386]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363