Detailed information NTG0847550

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QSV42_RS22580
• Genome accession: NZ_CP128466
• Coordinates: 4535100..4535900 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain 22KM2707
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4530100..4540900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QSV42_RS22545 (QSV42_22540)	gspH	4530253..4530768 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
QSV42_RS22550 (QSV42_22545)	gspI	4530765..4531130 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
QSV42_RS22555 (QSV42_22550)	gspJ	4531127..4531723 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
QSV42_RS22560 (QSV42_22555)	gspK	4531720..4532700 (+)	981	WP_077263807.1	type II secretion system minor pseudopilin GspK	-
QSV42_RS22565 (QSV42_22560)	gspL	4532693..4533889 (+)	1197	WP_117107499.1	type II secretion system protein GspL	-
QSV42_RS22570 (QSV42_22565)	-	4533882..4534367 (+)	486	WP_012968921.1	type II secretion system protein M	-
QSV42_RS22575 (QSV42_22570)	-	4534357..4535103 (+)	747	WP_012968922.1	type II secretion system protein N	-
QSV42_RS22580 (QSV42_22575)	pilD	4535100..4535900 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
QSV42_RS22585 (QSV42_22580)	hrpB	4535927..4538356 (-)	2430	WP_096834294.1	ATP-dependent helicase HrpB	-
QSV42_RS22590 (QSV42_22585)	thpR	4538429..4538965 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
QSV42_RS22595 (QSV42_22590)	sfsA	4538965..4539681 (+)	717	WP_072123497.1	DNA/RNA nuclease SfsA	-
QSV42_RS22600 (QSV42_22595)	dksA	4539844..4540299 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=847550 QSV42_RS22580 WP_008807384.1 4535100..4535900(+) (pilD) [Klebsiella variicola strain 22KM2707]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=847550 QSV42_RS22580 WP_008807384.1 4535100..4535900(+) (pilD) [Klebsiella variicola strain 22KM2707]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCACTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425