Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V4939_RS01385 Genome accession   NZ_CP144733
Coordinates   277160..278944 (-) Length   594 a.a.
NCBI ID   WP_012699166.1    Uniprot ID   C1DJF3
Organism   Azotobacter vinelandii DJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272160..283944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4939_RS01360 (V4939_01360) - 273013..273813 (-) 801 WP_012699159.1 ATP-binding cassette domain-containing protein -
  V4939_RS01365 (V4939_01365) - 273810..274958 (-) 1149 WP_012699160.1 ABC transporter permease -
  V4939_RS01370 (V4939_01370) - 275110..275781 (+) 672 WP_012699161.1 DUF480 domain-containing protein -
  V4939_RS01375 (V4939_01375) - 276390..276725 (-) 336 WP_012699164.1 DUF2388 domain-containing protein -
  V4939_RS01380 (V4939_01380) - 276814..277065 (-) 252 WP_012699165.1 membrane protein -
  V4939_RS01385 (V4939_01385) pilB 277160..278944 (-) 1785 WP_012699166.1 GspE/PulE family protein Machinery gene
  V4939_RS01390 (V4939_01390) - 279019..279708 (-) 690 WP_012699167.1 DUF502 domain-containing protein -
  V4939_RS01395 (V4939_01395) - 279761..281125 (-) 1365 WP_012699168.1 CYTH domain-containing protein -
  V4939_RS01400 (V4939_01400) - 281620..282639 (+) 1020 WP_012699169.1 transporter -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65526.17 Da        Isoelectric Point: 8.1757

>NTDB_id=847508 V4939_RS01385 WP_012699166.1 277160..278944(-) (pilB) [Azotobacter vinelandii DJ]
MPSIASPTEDRRLDPGELLRELVACGRIDRDSAERCLAIQRSTPDSRQHPLESLAAQRLDDRLRPGRKLDLESLTQWLAD
HAGQPYLRIDPLKLDVAAITPLMSRAFAQRHGILAVAVAADGVTVASAQPFVGAWEADLAQALRRPIRRVVANPVDIRRF
TQEFYRLARSVSGASAPEQKSAGTGNFEQLFRLGAADREPDANDAHIVTIVDWLLQYAFEQRASDIHIEPRREAGSVRFR
IDGVLHNVYRFPSQVSMAVVGRLKSLGRMNVAEKRKPQDGRVKTRSPDGGEIELRLSTLPTAFGEKLVMRIFDPEVLLKS
FDALGFSADDLRRWRSMTDQPNGIVLVTGPTGSGKTTTLYTTLKQLATPEVNVCTIEDPIEMIEPAFNQMQVQRNIDLDF
ASGVRALMRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPGAIARLLELGVPHYLIKATLLGVMAQRLVRT
LCPHCKAPVSLDAADWQALTRPWNAPPPSAAQRAVGCAECRDTSYRGRAGVYEIMLLNDALKALIKTDTDLLALRRAAFR
DGMRSLRLSGALKVAAGSTTLEEVMRVTPQSDRQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=847508 V4939_RS01385 WP_012699166.1 277160..278944(-) (pilB) [Azotobacter vinelandii DJ]
ATGCCTTCCATCGCATCTCCCACCGAGGACCGCCGGCTCGATCCGGGCGAGCTGTTGCGCGAGCTGGTCGCCTGCGGCCG
GATCGACCGGGACAGCGCCGAGCGCTGCCTGGCGATCCAGCGCAGCACCCCGGACAGCCGACAGCACCCACTCGAGTCGC
TCGCCGCCCAACGGCTCGACGACCGCCTCCGACCCGGCCGCAAGCTCGACCTGGAAAGCCTCACCCAGTGGCTGGCCGAC
CATGCCGGGCAACCCTACCTGCGTATCGACCCGCTGAAGCTCGACGTCGCCGCGATCACCCCGCTGATGTCCCGCGCCTT
CGCCCAGCGCCACGGCATCCTCGCCGTGGCGGTCGCGGCGGACGGCGTCACGGTCGCCAGCGCGCAACCTTTCGTCGGCG
CCTGGGAGGCCGATCTGGCCCAGGCACTCAGGCGGCCGATCCGCCGGGTGGTGGCCAACCCCGTCGACATCCGCCGCTTC
ACCCAGGAGTTCTACCGCCTGGCCAGGTCGGTCAGCGGCGCCTCGGCCCCGGAGCAGAAGAGCGCCGGCACCGGCAACTT
CGAGCAGTTGTTCAGGCTCGGCGCGGCGGACCGGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGCTCCAGTACGCCTTCGAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGGCCGGCAGCGTGCGCTTTCGC
ATCGACGGCGTGTTGCACAACGTCTACCGGTTCCCGTCGCAGGTGAGCATGGCGGTAGTCGGCCGGCTGAAGAGTCTCGG
CCGGATGAACGTCGCCGAGAAGCGCAAGCCGCAGGACGGCCGGGTCAAGACCAGAAGCCCGGACGGCGGCGAGATCGAGC
TGCGCCTCTCGACCCTGCCGACCGCCTTCGGCGAGAAGCTGGTGATGCGCATCTTCGACCCCGAGGTATTGCTCAAGAGC
TTCGACGCCCTCGGTTTTTCCGCCGACGACCTGCGGCGCTGGCGGAGCATGACCGACCAACCCAACGGCATCGTCCTGGT
CACCGGCCCGACCGGCTCGGGCAAGACCACCACCCTCTACACCACGCTGAAACAACTGGCGACGCCGGAAGTCAACGTCT
GCACCATCGAAGACCCCATCGAGATGATCGAACCGGCGTTCAACCAGATGCAGGTGCAGCGCAACATCGATCTGGACTTC
GCCAGCGGCGTGCGCGCGCTGATGCGCCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTGGAAACCGCCGA
GATGGCCATCCAGGCGGCACTCACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACGACGCGCCCGGCGCCATCGCCC
GCCTGCTCGAGCTGGGCGTGCCTCATTACCTGATCAAGGCCACCCTGCTCGGAGTCATGGCCCAGCGCCTGGTACGAACC
CTGTGCCCGCACTGCAAGGCGCCGGTCAGCCTCGATGCGGCCGACTGGCAGGCCCTCACCCGTCCCTGGAACGCCCCGCC
GCCGAGCGCTGCGCAGCGGGCGGTGGGCTGCGCCGAATGCCGCGACACCAGCTATCGCGGGCGCGCCGGAGTCTACGAGA
TCATGCTGCTGAACGATGCCCTCAAAGCGCTGATCAAAACCGATACCGACCTGCTCGCGCTGCGCCGCGCCGCCTTCAGG
GACGGCATGCGCAGCCTGCGTCTGTCCGGCGCGCTGAAGGTCGCCGCCGGCTCGACCACCCTCGAAGAAGTCATGCGCGT
CACCCCGCAGAGCGATCGGCAGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(215-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1DJF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.789

100

0.386
  pilF Thermus thermophilus HB27

37.394

99.495

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

35.17

100

0.365