Detailed information NTG0846916

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QR290_RS16395
• Genome accession: NZ_CP128260
• Coordinates: 3688471..3689580 (-)
• Length: 369 a.a.
• NCBI ID: WP_289203095.1
• Uniprot ID: -
• Organism: Pseudomonas fluorescens strain PH.SM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3683471..3694580

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QR290_RS16380 (QR290_16380)	-	3684273..3685664 (+)	1392	WP_085603294.1	GntP family permease	-
QR290_RS16385 (QR290_16385)	hbdH	3685672..3686445 (+)	774	WP_007952053.1	3-hydroxybutyrate dehydrogenase	-
QR290_RS16390 (QR290_16390)	-	3686509..3688464 (+)	1956	WP_289203094.1	acetoacetate--CoA ligase	-
QR290_RS16395 (QR290_16395)	pilU	3688471..3689580 (-)	1110	WP_289203095.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QR290_RS16400 (QR290_16400)	-	3689708..3692245 (+)	2538	WP_115078101.1	PAS domain-containing protein	-
QR290_RS16405 (QR290_16405)	-	3692296..3692571 (+)	276	WP_007952044.1	peptidylprolyl isomerase	-
QR290_RS16410 (QR290_16410)	-	3692621..3693556 (-)	936	WP_289203096.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41099.05 Da
• Isoelectric Point: 6.8358

>NTDB_id=846916 QR290_RS16395 WP_289203095.1 3688471..3689580(-) (pilU) [Pseudomonas fluorescens strain PH.SM]
MEIDALLKQLASRHGSDLFLSTGAPPSARFDGVLTPLSEQPFKPGEVATVAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLESVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNANQALDRI
INFFPEERRPQLLHALGNHLKAFVSQRLVRTRDGQRRAAAEVMLGTPTIADLIRRNDFGELKTIMEKSAELGMQTFDAAL
FALVAEGVIDEDEALKNADSVNNLRLRLKLHADTMPSPPPSTGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=846916 QR290_RS16395 WP_289203095.1 3688471..3689580(-) (pilU) [Pseudomonas fluorescens strain PH.SM]
ATGGAAATCGACGCACTACTCAAACAACTGGCGAGCCGCCACGGTTCGGATCTGTTCCTGTCCACGGGCGCACCGCCCAG
TGCGCGGTTCGACGGGGTGTTGACGCCGTTGAGCGAGCAGCCCTTCAAACCCGGTGAGGTGGCAACTGTCGCCGCCTCCC
TGATGGACGCCGAGCAGCGTCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCCCGCACCGGCATCGGCCGC
TTCCGGGTCAATATTTTCAAACAGCGCAACGATGTGTCGATCGTGATCCGCAACGTCAAGCTCGATATTCCGCGATTCGA
AGACCTGAAACTGCCGCCGGTATTACTCGAATCGGTGATGCTCAAGCAAGGCCTGATCCTGTTCGTCGGCGCCACCGATT
CGGGTAAATCCACGTCGCTGGCGGCGCTGATCGACCATCGCAATCGCCACAGCAGCGGGCACATCATCACCATTGAAGAT
CCGGTGGAATACATCCATCGCCACAAGAAATCGATCATCAACCAGCGGGAGGTCGGCGTCGATACCCGAAGTTTTCACGC
GGCATTGAAAAACACCTTGCGCCAAGCGCCGGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAGACCATGGAACATG
CGCTGGCCTTCGCCGAAACCGGGCATCTGGTGTTGTCGACATTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCCGAAGAGCGCCGGCCGCAATTGCTGCATGCCTTGGGCAATCACCTCAAGGCGTTCGTCTCCCAGCG
TCTGGTCCGAACCCGGGACGGTCAGCGACGGGCCGCCGCCGAGGTCATGCTCGGTACGCCGACGATTGCCGACCTGATCC
GGCGCAATGACTTTGGCGAGTTGAAAACCATCATGGAAAAATCGGCGGAACTGGGCATGCAGACCTTTGACGCTGCACTG
TTCGCGCTGGTGGCAGAAGGGGTGATCGATGAAGACGAGGCCCTGAAAAACGCGGATTCGGTGAACAACCTGCGCCTGCG
TCTGAAGCTGCATGCCGATACCATGCCGTCCCCACCGCCCTCGACCGGCGAGTGGGGACTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	96.206	0.542
pilU	Acinetobacter baylyi ADP1	53.977	95.393	0.515
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.118	91.87	0.369
pilT	Pseudomonas aeruginosa PAK	39.244	93.225	0.366
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363