Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QSU96_RS02010 Genome accession   NZ_CP128200
Coordinates   415741..416847 (+) Length   368 a.a.
NCBI ID   WP_286279820.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFBJ07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410741..421847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSU96_RS01975 (QSU96_01975) - 411092..411691 (-) 600 WP_232658421.1 XTP/dITP diphosphatase -
  QSU96_RS01980 (QSU96_01980) - 411715..412146 (-) 432 WP_014205827.1 DUF4426 domain-containing protein -
  QSU96_RS01985 (QSU96_01985) yggU 412204..412494 (-) 291 WP_004724263.1 DUF167 family protein YggU -
  QSU96_RS01990 (QSU96_01990) - 412494..413051 (-) 558 WP_004724262.1 YggT family protein -
  QSU96_RS01995 (QSU96_01995) proC 413103..413921 (-) 819 WP_286279818.1 pyrroline-5-carboxylate reductase -
  QSU96_RS02000 (QSU96_02000) - 413959..414666 (-) 708 WP_286279819.1 YggS family pyridoxal phosphate-dependent enzyme -
  QSU96_RS02005 (QSU96_02005) pilT 414692..415729 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  QSU96_RS02010 (QSU96_02010) pilU 415741..416847 (+) 1107 WP_286279820.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QSU96_RS02015 (QSU96_02015) ruvX 416944..417369 (-) 426 WP_055467261.1 Holliday junction resolvase RuvX -
  QSU96_RS02020 (QSU96_02020) - 417432..417995 (-) 564 WP_004724256.1 YqgE/AlgH family protein -
  QSU96_RS02025 (QSU96_02025) gshB 418034..418990 (-) 957 WP_004724255.1 glutathione synthase -
  QSU96_RS02030 (QSU96_02030) rsmE 419003..419734 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QSU96_RS02035 (QSU96_02035) - 419842..420543 (-) 702 WP_004724253.1 endonuclease -
  QSU96_RS02040 (QSU96_02040) - 420682..421182 (-) 501 WP_004724252.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41339.37 Da        Isoelectric Point: 6.5588

>NTDB_id=846712 QSU96_RS02010 WP_286279820.1 415741..416847(+) (pilU) [Vibrio furnissii strain VFBJ07]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVRTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTIAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKTQRGDVTSSGSLDHVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=846712 QSU96_RS02010 WP_286279820.1 415741..416847(+) (pilU) [Vibrio furnissii strain VFBJ07]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTGTATATCACTGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAATTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCAGGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGCTTTCGC
GTCAGTGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGTATTGAAACGCAAATTCCCACGTTTGATGAACT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGTTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCCATGACCGGGTATCGTAATATGCATCGCACCGGGCATATTCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGTGAAGTGGGGTTGGACACTGAAAGTTATGAAGTCGCACT
GAAGAACTCGCTGCGTCAGGCACCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCGATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCGCTTGAACGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGGGTTGTCGGCCAGCAACTGAT
CCGCGATAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GTGGCGAGCTGCATGAGTTGAAAAACACCATCGCGAAATCAAAAGAGGTCGGTATGCAGACGTTTGATCAGGCGTTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGTGAGCAAGATGCGCTGCACAGTGCGGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGACGCAGCGGGGCGATGTGACCAGCAGCGGGTCGCTGGATCATGTGAAAATCGATATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.87

100

0.859
  pilU Pseudomonas stutzeri DSM 10701

56.857

95.109

0.541
  pilU Acinetobacter baylyi ADP1

54.131

95.38

0.516
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.769

88.315

0.378
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.12

0.364
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361