Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   QRE67_RS05620 Genome accession   NZ_CP128153
Coordinates   1135754..1139479 (+) Length   1241 a.a.
NCBI ID   WP_286123918.1    Uniprot ID   -
Organism   Bacillus sp. DX3.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1130754..1144479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRE67_RS05610 (QRE67_05605) lepB 1131286..1131849 (+) 564 WP_286123916.1 signal peptidase I -
  QRE67_RS05615 (QRE67_05610) addB 1132245..1135757 (+) 3513 WP_286123917.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QRE67_RS05620 (QRE67_05615) addA 1135754..1139479 (+) 3726 WP_286123918.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QRE67_RS05625 (QRE67_05620) gerPF 1139582..1139797 (-) 216 WP_286123919.1 spore germination protein GerPF -
  QRE67_RS05630 (QRE67_05625) - 1139849..1140229 (-) 381 WP_286123920.1 spore germination protein GerPE -
  QRE67_RS05635 (QRE67_05630) - 1140242..1140436 (-) 195 WP_286123921.1 spore gernimation protein GerPD -
  QRE67_RS05640 (QRE67_05635) gerPC 1140443..1141057 (-) 615 WP_286123922.1 spore germination protein GerPC -
  QRE67_RS05645 (QRE67_05640) - 1141126..1141332 (-) 207 WP_286123924.1 spore germination protein GerPB -
  QRE67_RS05650 (QRE67_05645) - 1141349..1141570 (-) 222 WP_286123925.1 spore germination protein -
  QRE67_RS05655 (QRE67_05650) - 1141867..1142046 (-) 180 WP_286123926.1 aspartyl-phosphate phosphatase Spo0E family protein -
  QRE67_RS05660 (QRE67_05655) - 1142290..1143189 (+) 900 WP_286125196.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142858.18 Da        Isoelectric Point: 5.1286

>NTDB_id=846335 QRE67_RS05620 WP_286123918.1 1135754..1139479(+) (addA) [Bacillus sp. DX3.1]
MMQNWPVKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIRKIISTENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDDPASQHVRKQLSLLNKASISTIHSFCLQVIRTYYYMLDIDPRFRIANQTENELLKEEVLDDILEEEYGIE
GNDIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPALWLDKLVEAYDVEGKSIEDLTYASYLLEDVKFQLRAAEQHV
RKAIELAMLPDGPAPRIETLQADLALLTTLSAASHQSWTSVYEAMQTISWQTLKRIKKSDYNDDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLQKLVQLVKVFTGRFQTIKRDKGMVDFTDLEHFCLQILSEHTENGEMYPSPVALQ
YRNKFAEVLVDEYQDTNFVQESIIKLVTKDSEREGNLFMVGDVKQSVYRFRLAEPGLFLGKYKRFTSEGIDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEAVGEIDYDADAELKLGASYPAGDDVAAELLCIHQSDKEVSDGEEGVEVEKAQLEARLI
AQRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVNIMMNIFRVIDN
PMQDIPLAAVLRSPIVGLHDEELAMLRAHEKKGSFYEVMRSFLQGAPLEEEAELHRKLQWFYDLLQGWREFARQQSLSDL
IWKVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIRRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDKDKEIEKWLDAREHTDWLLPDYVRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPNDIYQYDASWK
VEAIDGASLQAPEPIQEEKQELLEALREKKPVPVESERKEEVYARLTWQYAYGEATAHRAKQSVTEIKRNYQSEDGSDHA
FIKKIRAPIQTRPRFMEKKGLSYAERGTAVHAVMQHVNLKKPITAETIQEQIAEMVNKELLTFEQAEEIAAERIVAFFDT
ELGKRVLAAKSVEREVPFTMMLSAEEAYQNWQGEHEESVLVQGVIDCMMEEEDGVVLVDFKTDTIEGRFPGGFDQARPVL
EDRYKVQLTLYGKALEKSLGQPVKGKYLYFFDGNHVLRVDE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=846335 QRE67_RS05620 WP_286123918.1 1135754..1139479(+) (addA) [Bacillus sp. DX3.1]
ATGATGCAAAATTGGCCAGTAAAACCAGAAGGTAGTCAATGGACCGATGATCAATGGAAGGCAGTTGTTGCGAATGGTCG
TGATATTTTAGTGGCAGCGGCAGCCGGATCAGGAAAAACGGCTGTACTTGTAGAGCGGATGATCCGTAAAATTATTTCTA
CTGAGAATCCTGTGGATGTAGATCGCTTACTTGTTGTGACGTTTACAAACGCAGCAGCACAAGAGATGAAAAACAGAATC
GGTGAAGCATTAGAAAAAGTATTGATTGATGATCCAGCGTCACAACACGTCCGTAAACAACTGAGTTTATTAAATAAAGC
ATCGATTTCAACAATTCATTCATTCTGTTTGCAAGTCATTCGAACGTACTATTATATGCTCGATATTGATCCGCGTTTTC
GCATTGCCAACCAGACAGAAAACGAATTATTAAAAGAAGAAGTACTAGACGATATATTAGAAGAAGAGTATGGAATCGAA
GGAAATGACATCTTCTTTGAACTTGTCGATCGTTATACGAGTGATCGAAGCGATGACGATTTACAGCGCATGATTTTAGC
GCTTCATACAGAGTCTAGAGCGCATCCGAATCCAGCGCTTTGGCTTGATAAACTTGTAGAAGCATATGATGTAGAAGGAA
AATCAATTGAAGACTTGACCTATGCCTCTTATTTATTAGAAGATGTGAAGTTTCAGCTTCGGGCGGCAGAGCAACATGTT
CGAAAAGCAATTGAGCTAGCGATGCTGCCAGATGGTCCTGCACCACGCATTGAGACATTGCAAGCGGATCTTGCGCTACT
CACGACGTTGTCTGCAGCGTCACATCAGTCTTGGACAAGTGTATATGAAGCAATGCAAACTATTTCTTGGCAAACATTAA
AGCGCATTAAGAAGAGTGACTATAATGATGATGTTGTGAAACAAGTAGATTCATTACGGAATAAAGCAAAAGATGAAGTG
AAGAAGCTGCAAGAGGAGTTATTTAGCCGTAAACCAGAAAGCTTTTTACGTGATTTTCAAGATATGCACCCTGTGCTGCA
AAAGCTTGTTCAGCTTGTAAAAGTATTTACAGGGCGTTTTCAAACGATAAAGCGTGATAAAGGAATGGTTGATTTTACAG
ATTTAGAGCATTTTTGTTTGCAGATTTTAAGTGAACACACTGAGAACGGTGAAATGTATCCATCGCCAGTTGCGCTTCAA
TATCGTAATAAGTTTGCGGAAGTTCTTGTTGATGAATATCAAGATACGAACTTTGTGCAGGAGTCGATTATTAAACTTGT
AACGAAAGATTCAGAGCGAGAAGGTAATTTATTTATGGTCGGCGACGTAAAGCAATCTGTTTATCGGTTTCGATTAGCAG
AACCTGGCTTGTTTTTAGGAAAATATAAACGTTTTACGTCTGAAGGAATAGACGGCGGTATGAAAATTGATTTAGCGAAA
AACTTCCGAAGTCGTCATGAAGTGTTAGCTGGCACGAACTTTATTTTTAAACAGATTATGGGCGAAGCAGTTGGGGAAAT
TGACTATGATGCGGATGCAGAGTTAAAGCTAGGAGCGAGTTATCCAGCAGGTGACGATGTCGCAGCCGAACTGTTATGTA
TTCATCAATCTGATAAAGAAGTGTCAGATGGAGAAGAAGGTGTGGAAGTCGAAAAAGCGCAACTAGAAGCACGTCTCATT
GCACAAAGAATAAAGGCGATGGTTGATTCCGGATATGAAGTATATGACCGTAAAACAGATAGTATGCGCCCTGTGAAATA
TCGTGATTTTGTTATTTTACTTCGTTCGATGCCATGGGCACCGCAAATTATGGAAGAGTTGAAATTACAAGGTATTCCTG
TTTATGCAGAACTAGCGACAGGTTATTTTGAAGCGACAGAAGTGAACATCATGATGAATATCTTTCGGGTGATTGACAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTGCGCTCGCCGATTGTCGGATTACATGATGAGGAACTCGCGATGTTACG
TGCTCATGAAAAGAAAGGTTCGTTTTATGAAGTAATGCGCTCGTTCTTACAAGGTGCACCGCTCGAGGAAGAGGCAGAGT
TACACAGGAAATTACAATGGTTTTATGATCTCCTGCAAGGCTGGCGAGAATTTGCCCGTCAGCAGTCGCTTTCTGATCTC
ATTTGGAAAGTATATCGTGAAACAGGTTATTATGATTTTGTCGGTGGTCTTCCAGCAGGAAAGCAGCGTCAGGCCAATTT
ACGTGTGTTATATGATCGTGCTCGTCAATATGAAGCAACGTCTTTCCGCGGGTTATTCCGTTTTCTGCGTTTTATTGAAC
GGATTTTAGAGCGCGGTGATGATATGGGAACAGCAAGAGCGCTTGGTGAACAAGAAGATGTTGTCCGCATTATGACCATT
CATAAAAGTAAAGGGTTAGAGTTTCCGGTTGTATTCGTAGCGGGACTAGGGCGCCGCTTTAATACGCAAGATTTAATGAA
GCGCTTCTTATTACACAAAGATTTCGGTTTCGGATCGCAATTTATTGATCCGCGTAAACGAATTAAATATACGACGCTAT
CGCAGTTGGCAATTAGGCGGAAAATGAAGATGGAACTGATTGCCGAAGAAATGCGAGTATTATATGTTGCGTTAACACGT
GCAAAAGAAAAACTCATTTTAATCGGTACGGTGAAGGATAAAGACAAAGAGATAGAAAAGTGGCTGGATGCACGGGAACA
TACCGATTGGTTGTTACCAGATTATGTTCGCGCGGGAGCATCTTGTTATTTAGATTGGATTGCACCATCATTATATAGGC
ACCGTGATAGTGAAATGCTTCTTGAACTTGGACAGGGAAACATTCCAAATGATATTTATCAGTATGACGCAAGCTGGAAA
GTTGAGGCTATTGATGGTGCAAGTTTGCAGGCGCCAGAACCAATTCAAGAAGAAAAACAAGAACTATTAGAAGCACTTCG
TGAGAAGAAGCCTGTTCCAGTAGAGAGCGAACGAAAAGAAGAAGTATACGCGCGGTTAACATGGCAATATGCATATGGAG
AAGCAACAGCACATCGAGCAAAACAGTCTGTCACGGAAATTAAGCGAAACTATCAATCGGAAGATGGAAGTGATCATGCT
TTTATTAAAAAGATACGTGCGCCTATCCAAACGCGCCCGCGGTTTATGGAGAAAAAAGGACTGAGTTATGCGGAACGTGG
AACAGCTGTACACGCTGTGATGCAGCATGTGAATTTGAAAAAGCCAATTACAGCAGAAACAATTCAAGAACAAATTGCTG
AAATGGTGAATAAAGAATTGTTAACATTTGAGCAGGCAGAAGAGATTGCAGCTGAGAGAATCGTTGCTTTCTTTGATACG
GAGCTTGGGAAACGGGTACTGGCTGCGAAAAGTGTAGAACGCGAAGTGCCATTTACAATGATGTTATCAGCAGAGGAAGC
GTATCAAAATTGGCAAGGAGAGCATGAAGAATCTGTGCTTGTGCAAGGTGTCATCGATTGCATGATGGAAGAGGAAGACG
GCGTTGTATTAGTTGATTTTAAAACTGATACCATTGAAGGGCGTTTTCCAGGTGGATTTGATCAGGCAAGACCTGTTTTA
GAAGATCGCTATAAAGTACAGCTTACATTGTATGGAAAGGCACTGGAGAAAAGTTTAGGTCAGCCTGTGAAGGGAAAATA
TTTATACTTTTTTGATGGAAATCATGTGCTACGAGTAGATGAGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1059-1231)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

54.032

99.919

0.54