Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QRE67_RS05615 Genome accession   NZ_CP128153
Coordinates   1132245..1135757 (+) Length   1170 a.a.
NCBI ID   WP_286123917.1    Uniprot ID   -
Organism   Bacillus sp. DX3.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1127245..1140757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRE67_RS05590 (QRE67_05585) aceA 1127246..1128523 (+) 1278 WP_286123913.1 isocitrate lyase -
  QRE67_RS05595 (QRE67_05590) - 1128955..1129158 (+) 204 WP_003195857.1 cold-shock protein -
  QRE67_RS05600 (QRE67_05595) - 1129670..1130248 (+) 579 WP_286123914.1 competence protein ComK -
  QRE67_RS05605 (QRE67_05600) - 1130635..1131228 (+) 594 WP_286123915.1 TVP38/TMEM64 family protein -
  QRE67_RS05610 (QRE67_05605) lepB 1131286..1131849 (+) 564 WP_286123916.1 signal peptidase I -
  QRE67_RS05615 (QRE67_05610) addB 1132245..1135757 (+) 3513 WP_286123917.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QRE67_RS05620 (QRE67_05615) addA 1135754..1139479 (+) 3726 WP_286123918.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QRE67_RS05625 (QRE67_05620) gerPF 1139582..1139797 (-) 216 WP_286123919.1 spore germination protein GerPF -
  QRE67_RS05630 (QRE67_05625) - 1139849..1140229 (-) 381 WP_286123920.1 spore germination protein GerPE -
  QRE67_RS05635 (QRE67_05630) - 1140242..1140436 (-) 195 WP_286123921.1 spore gernimation protein GerPD -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134389.56 Da        Isoelectric Point: 5.1676

>NTDB_id=846334 QRE67_RS05615 WP_286123917.1 1132245..1135757(+) (addB) [Bacillus sp. DX3.1]
MSLRFVIGRAGSGKSAFCLREVQEELKQCPRGETILYLVPEQMTFQTQQALIGNGDVSSSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVEARKEGLYVFQKAAEQNGFFEHLGSMIAEFKRYNLTPSNVYEMWQQLDTHSNGNEQKILA
NKMYDLQILYDDFERALIGKYLDSEDYLQLLVEKLPHSDYVNGAEIYIDGFHSFSPQELEIIRQLLRCGARITITLTLDE
KTLSGPVSELDLFYQTILTYEKIKQLAHEEKITIENTIPMREQPRFRSAALAHLEAQYESRPNAKFQGEPNITLYTAANL
RAEVEGVAREVRRLVAEEEYRYRDIAVLLRNGESYYDVMKTLFTDYNIPHFIDEKRPMSHHPLIECIRSALETISGNWRY
DAVFRCVKTELLYPLDVRKEIMREEMDEFENYCLAYGVQGKRWTADEPWTYRRYRSLDGVNGMQTDSEREMEEKINRLRD
VIRTPIIRLQKRLKRAGTTMQMCEAVYLFLEELDVPKKLEDLRMRAEAEGNFLFANDHDQVWEEVMKLLDTCVEMIGEEK
MSLAMFIDVMTTGLEALQFANIPPSLDQVLIANIDHSRLSDIRAAFVIGANEGIIPAAPSDEGMLSDEEREVLVSAGVEL
APTTRQTLLEEQFIIYQTVTRASEKLYISCPLADEEGKTLLSSSFIKKVKRMFPFVKEVFITNDVNDLSRHEQISYVATP
EVTLSYVTQQLQNWKRYGFEGNLDFWWDVYNFYVTTPAWKQKSYRVLSSLFYRNQAQKLSAVVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWSDLSIKECEHLSVMVIEEIAPLLQRQIL
LSSNRHYYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSAKALNLTEVYYGLALQMLTYLDVVISNANTWMKKDGTASPAGVLYFHIHNPIVEMKGDASEEEIEREIL
KKFKMKGLVLGDTDVVRLMDNKLVTGSSDIISAGLKKDGSFSARSDIASEQEFTVLQQYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFRSVCQFDESLEDNQYRTLKDMKDSEAMEKMREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=846334 QRE67_RS05615 WP_286123917.1 1132245..1135757(+) (addB) [Bacillus sp. DX3.1]
ATGTCGCTTCGATTTGTGATTGGACGAGCTGGAAGTGGAAAAAGTGCATTTTGTTTACGTGAAGTGCAAGAAGAACTTAA
ACAGTGTCCAAGAGGTGAAACAATTTTATATCTTGTGCCAGAGCAGATGACGTTCCAAACGCAGCAGGCGTTAATCGGTA
ATGGGGACGTAAGTAGCTCGATTCGGGCGCAAGTTTTTAGTTTTTCACGTTTAGCGTGGAAGGTTCTGCAAGAGGTTGGT
GGTGCAAGTCGTCTTCATATTGATGAGGCGGGTGTGCACATGTTGCTTCGCAAAATTGTTGAGGCACGTAAAGAAGGATT
ATATGTGTTTCAAAAAGCCGCGGAACAAAATGGCTTTTTTGAGCACCTTGGCAGCATGATTGCTGAATTTAAACGTTATA
ACTTAACACCATCTAATGTATATGAAATGTGGCAGCAGTTAGATACTCATAGCAATGGCAATGAGCAAAAAATATTAGCG
AATAAAATGTACGATCTTCAGATTTTGTACGATGATTTTGAACGAGCTTTAATAGGTAAATATTTAGATTCTGAAGATTA
TTTACAGTTGCTTGTGGAGAAACTTCCGCATTCTGATTATGTAAATGGTGCTGAGATTTATATAGATGGATTTCATTCGT
TTTCACCGCAAGAATTAGAGATTATTAGGCAGCTTCTTAGATGCGGGGCAAGAATAACGATTACATTAACATTGGATGAA
AAAACATTGTCTGGGCCGGTGAGCGAACTTGATTTGTTTTATCAAACAATTCTTACGTATGAAAAGATAAAACAGCTGGC
ACACGAGGAGAAAATTACGATTGAAAATACGATTCCGATGCGAGAGCAACCGCGGTTTCGTTCAGCTGCATTGGCGCATT
TAGAAGCACAATATGAATCTCGTCCAAATGCAAAATTTCAAGGTGAACCTAATATTACACTGTATACAGCGGCTAACTTA
CGAGCAGAAGTCGAAGGGGTTGCCCGTGAAGTGCGAAGACTTGTTGCAGAGGAGGAGTATCGCTACCGCGATATTGCTGT
TCTTCTTCGTAACGGAGAAAGCTACTATGATGTGATGAAAACCCTTTTTACCGATTATAATATTCCACACTTTATTGATG
AAAAGCGTCCGATGTCCCATCATCCGCTCATTGAATGCATTCGTTCTGCGCTGGAAACCATTAGTGGAAATTGGCGCTAT
GATGCAGTATTTCGCTGTGTGAAAACGGAGTTGTTATATCCGTTAGATGTAAGAAAAGAAATAATGCGTGAAGAGATGGA
TGAATTTGAAAATTATTGTTTAGCGTACGGTGTGCAAGGAAAGAGATGGACAGCGGATGAACCGTGGACATATCGCCGTT
ACCGTTCGTTAGATGGAGTAAATGGAATGCAAACCGATAGCGAGCGCGAGATGGAAGAAAAAATAAATCGTCTTCGTGAT
GTTATACGGACACCAATTATTCGTTTGCAGAAGCGTTTAAAACGTGCTGGAACGACAATGCAAATGTGTGAAGCGGTTTA
TTTATTTTTAGAAGAACTGGACGTTCCGAAAAAACTGGAAGACTTACGCATGCGGGCCGAAGCAGAAGGAAACTTCTTAT
TTGCAAATGATCATGATCAAGTATGGGAAGAAGTGATGAAGCTTCTTGATACATGTGTGGAAATGATCGGTGAGGAAAAG
ATGTCATTGGCTATGTTTATCGATGTGATGACAACTGGTTTAGAGGCGCTTCAGTTTGCCAATATCCCGCCATCTTTAGA
TCAAGTACTAATTGCCAATATTGATCATTCTCGTTTATCGGATATCCGAGCGGCCTTTGTTATCGGGGCCAATGAAGGAA
TTATTCCAGCAGCGCCTTCAGACGAGGGTATGTTATCAGATGAAGAGCGAGAGGTTCTCGTTTCAGCTGGTGTAGAATTA
GCACCGACAACAAGACAGACTTTATTAGAAGAACAATTTATCATTTATCAAACGGTGACACGTGCATCCGAAAAGTTATA
TATTTCTTGTCCACTTGCCGATGAAGAAGGGAAAACATTACTCTCATCTAGTTTTATTAAGAAAGTAAAAAGAATGTTCC
CTTTTGTCAAAGAAGTATTTATAACAAATGATGTGAACGATTTGTCTCGTCATGAGCAAATTTCGTATGTAGCAACGCCG
GAGGTTACGCTGTCGTATGTGACGCAGCAGCTGCAAAACTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTATGTTACAACACCTGCGTGGAAGCAAAAAAGTTATCGTGTGTTATCCAGTTTGTTCTATCGAA
ACCAAGCACAGAAATTAAGCGCGGTTGTGAGCCGAGACTTGTACGGTGATACAATTAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAATCGCTGTGCCTATGCTCATTTCGCGCAGCATGGGTTATCGCTAAGAGAGCGGGATATTTTTAAACTAGATGC
ACCGGATATCGGTGAACTATTCCATGCGGCATTAAAGAAAATTGCAGACAAGTTATTGCGTGAAAATCGCACTTGGTCTG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTCATGGTAATAGAAGAAATTGCACCGCTACTACAAAGGCAAATTCTA
TTAAGTTCAAATCGTCATTATTATTTAAAGCAAAAACTGCAGCAAATCATTTTCCGGACGTCTCTTATCCTTCGTGAGCA
TGCAAAATCAAGCGGGTTCGTACCGGTTGATTTAGAGGTGCCATTTGGTATGGGGGGGACAGGATCATTACCACCGATGG
AGTTTTCATTGCCGAACGGAGTGAAAATGGAAGTTGTTGGCCGCATTGACCGCGTAGATAAAGCGGAAGACGAGAGCGGT
ACGTTCCTGCGCATTATTGACTATAAGTCAAGTGCCAAAGCATTAAATCTAACAGAAGTGTATTATGGTTTAGCGCTACA
AATGTTAACGTATTTAGATGTTGTTATTTCTAATGCAAATACATGGATGAAGAAAGATGGGACGGCATCACCTGCTGGGG
TCCTTTATTTCCATATTCATAATCCAATTGTAGAAATGAAAGGCGACGCTTCTGAGGAAGAAATTGAGCGAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTTGTGCTCGGAGATACAGATGTTGTGCGTTTAATGGATAACAAACTTGTGACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGATGGAAGCTTTAGTGCTCGTTCTGATATTGCAAGTGAGCAAGAGTTTA
CGGTCTTGCAGCAATATGTGCATCATACATTTGAAAACATCGGAAAAGACATTACAGAAGGTGTAATTGATATTGCACCG
TATAAAATGGGGAATAAAGCAGCTTGTACGTTTTGTAATTTCCGCTCTGTCTGTCAGTTTGATGAATCGCTTGAAGATAA
TCAGTATCGTACGTTAAAAGATATGAAAGATAGTGAAGCAATGGAGAAAATGAGAGAGGAGGTTGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(301-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.145

100

0.491