Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QRD88_RS11385 Genome accession   NZ_CP128114
Coordinates   2212520..2213590 (-) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus safensis strain G6S3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2207520..2218590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD88_RS11335 (QRD88_11330) - 2207614..2208186 (-) 573 WP_024422971.1 signal peptidase I SipW -
  QRD88_RS11340 (QRD88_11335) tapA 2208239..2208766 (-) 528 WP_024422972.1 amyloid fiber anchoring/assembly protein TapA -
  QRD88_RS11345 (QRD88_11340) - 2209066..2209344 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  QRD88_RS11350 (QRD88_11345) - 2209379..2209573 (-) 195 WP_056767455.1 YqzE family protein -
  QRD88_RS11355 (QRD88_11350) comGG 2209633..2210016 (-) 384 WP_125586574.1 competence type IV pilus minor pilin ComGG -
  QRD88_RS11360 (QRD88_11355) comGF 2210013..2210540 (-) 528 WP_311164128.1 competence type IV pilus minor pilin ComGF -
  QRD88_RS11365 (QRD88_11360) comGE 2210452..2210766 (-) 315 WP_024427988.1 competence type IV pilus minor pilin ComGE -
  QRD88_RS11370 (QRD88_11365) comGD 2210750..2211196 (-) 447 WP_268422516.1 competence type IV pilus minor pilin ComGD -
  QRD88_RS11375 (QRD88_11370) comGC 2211189..2211482 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  QRD88_RS11380 (QRD88_11375) comGB 2211499..2212539 (-) 1041 WP_024422978.1 competence type IV pilus assembly protein ComGB -
  QRD88_RS11385 (QRD88_11380) comGA 2212520..2213590 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  QRD88_RS11390 (QRD88_11385) - 2213768..2214643 (-) 876 WP_024422979.1 STAS domain-containing protein -
  QRD88_RS11400 (QRD88_11395) - 2214867..2215247 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QRD88_RS11405 (QRD88_11400) - 2215460..2215702 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  QRD88_RS11410 (QRD88_11405) - 2215750..2216388 (-) 639 WP_034621600.1 MBL fold metallo-hydrolase -
  QRD88_RS11415 (QRD88_11410) - 2216546..2216719 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  QRD88_RS11420 (QRD88_11415) - 2216943..2217257 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  QRD88_RS11425 (QRD88_11420) - 2217334..2218587 (-) 1254 WP_286098631.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=845969 QRD88_RS11385 WP_025092972.1 2212520..2213590(-) (comGA) [Bacillus safensis strain G6S3]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=845969 QRD88_RS11385 WP_025092972.1 2212520..2213590(-) (comGA) [Bacillus safensis strain G6S3]
TTGTATGGGATTGAATATTTAAGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCAGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCGCTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTTGGAAAAGAGCTTGGTGAGTGTATCAAAGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643