Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QRD87_RS12260 Genome accession   NZ_CP128109
Coordinates   2343860..2344930 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus altitudinis strain D9_B_49     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338860..2349930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD87_RS12210 (QRD87_12210) - 2338955..2339527 (-) 573 WP_017357979.1 signal peptidase I SipW -
  QRD87_RS12215 (QRD87_12215) tapA 2339577..2340104 (-) 528 WP_061419578.1 amyloid fiber anchoring/assembly protein TapA -
  QRD87_RS12220 (QRD87_12220) - 2340357..2340683 (+) 327 WP_041506959.1 DUF3889 domain-containing protein -
  QRD87_RS12225 (QRD87_12225) - 2340718..2340912 (-) 195 WP_008342191.1 YqzE family protein -
  QRD87_RS12230 (QRD87_12230) comGG 2340973..2341356 (-) 384 WP_041506958.1 competence type IV pilus minor pilin ComGG -
  QRD87_RS12235 (QRD87_12235) comGF 2341353..2341850 (-) 498 WP_286108396.1 competence type IV pilus minor pilin ComGF -
  QRD87_RS12240 (QRD87_12240) comGE 2341792..2342106 (-) 315 WP_058214150.1 competence type IV pilus minor pilin ComGE -
  QRD87_RS12245 (QRD87_12245) comGD 2342090..2342536 (-) 447 WP_057079312.1 competence type IV pilus minor pilin ComGD -
  QRD87_RS12250 (QRD87_12250) comGC 2342529..2342822 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  QRD87_RS12255 (QRD87_12255) comGB 2342839..2343879 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  QRD87_RS12260 (QRD87_12260) comGA 2343860..2344930 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  QRD87_RS12265 (QRD87_12265) - 2345387..2346259 (-) 873 WP_041506953.1 STAS domain-containing protein -
  QRD87_RS12275 (QRD87_12275) - 2346484..2346864 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QRD87_RS12280 (QRD87_12280) - 2347077..2347319 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  QRD87_RS12285 (QRD87_12285) - 2347368..2348006 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  QRD87_RS12290 (QRD87_12290) - 2348167..2348340 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  QRD87_RS12295 (QRD87_12295) - 2348365..2348679 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=845856 QRD87_RS12260 WP_024718839.1 2343860..2344930(-) (comGA) [Bacillus altitudinis strain D9_B_49]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=845856 QRD87_RS12260 WP_024718839.1 2343860..2344930(-) (comGA) [Bacillus altitudinis strain D9_B_49]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCAGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649