Detailed information NTG0084558

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: JQN54_RS00085
• Genome accession: NZ_AP024305
• Coordinates: 17807..20476 (-)
• Length: 889 a.a.
• NCBI ID: WP_143590146.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain HB5008
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 12807..25476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQN54_RS00065 (TthHB5008_00100)	-	13653..14285 (-)	633	WP_008634004.1	histidine phosphatase family protein	-
JQN54_RS00070 (TthHB5008_00110)	purM	14282..15283 (-)	1002	WP_143590140.1	phosphoribosylformylglycinamidine cyclo-ligase	-
JQN54_RS00075 (TthHB5008_00120)	gatB	15289..16698 (-)	1410	WP_024119046.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
JQN54_RS00080 (TthHB5008_00130)	pilT	16709..17794 (-)	1086	WP_011173990.1	type IV pilus twitching motility protein PilT	Machinery gene
JQN54_RS00085 (TthHB5008_00140)	pilF	17807..20476 (-)	2670	WP_143590146.1	type IV pilus assembly ATPase PilB	Machinery gene
JQN54_RS00090 (TthHB5008_00150)	-	20473..20958 (-)	486	WP_011173992.1	YqeG family HAD IIIA-type phosphatase	-
JQN54_RS00095 (TthHB5008_00160)	pgeF	20993..21721 (-)	729	WP_011173993.1	peptidoglycan editing factor PgeF	-
JQN54_RS00100 (TthHB5008_00170)	surE	21718..22473 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
JQN54_RS00105 (TthHB5008_00180)	-	22532..22738 (-)	207	WP_011173995.1	cold-shock protein	-
JQN54_RS00110 (TthHB5008_00190)	-	22895..24184 (+)	1290	WP_011173996.1	ABC transporter substrate-binding protein	-
JQN54_RS00115 (TthHB5008_00200)	-	24236..25111 (+)	876	WP_011173997.1	carbohydrate ABC transporter permease	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98257.39 Da
• Isoelectric Point: 5.3348

>NTDB_id=84558 JQN54_RS00085 WP_143590146.1 17807..20476(-) (pilF) [Thermus thermophilus strain HB5008]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAALLL
LRSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLTLPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYIDPEEDPP
DPGAPLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNYEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQAEEEVPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=84558 JQN54_RS00085 WP_143590146.1 17807..20476(-) (pilF) [Thermus thermophilus strain HB5008]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCCTCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCACCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTG
AAGGAGGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTATATTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCCCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGA
ACTACGAAGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGCGGAGGAGGAGGTCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAAAACACCCAGACCATTGAAGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II/IV secretion system protein (T2SSE) (513-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	99.775	100	0.998
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.341	100	0.565