Detailed information NTG0844988

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QRD21_RS22720
• Genome accession: NZ_CP127873
• Coordinates: 4860597..4861394 (+)
• Length: 265 a.a.
• NCBI ID: WP_286114734.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain ELP1.34B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4855597..4866394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRD21_RS22685 (QRD21_22685)	gspH	4855738..4856250 (+)	513	WP_046877779.1	type II secretion system minor pseudopilin GspH	-
QRD21_RS22690 (QRD21_22690)	gspI	4856247..4856612 (+)	366	WP_046877780.1	type II secretion system minor pseudopilin GspI	-
QRD21_RS22695 (QRD21_22695)	gspJ	4856609..4857205 (+)	597	WP_046877781.1	type II secretion system minor pseudopilin GspJ	-
QRD21_RS22700 (QRD21_22700)	gspK	4857202..4858182 (+)	981	WP_014227974.1	type II secretion system minor pseudopilin GspK	-
QRD21_RS22705 (QRD21_22705)	gspL	4858175..4859368 (+)	1194	WP_286114733.1	type II secretion system protein GspL	-
QRD21_RS22710 (QRD21_22710)	-	4859361..4859846 (+)	486	WP_032748031.1	type II secretion system protein M	-
QRD21_RS22715 (QRD21_22715)	-	4859836..4860594 (+)	759	WP_196546365.1	type II secretion system protein N	-
QRD21_RS22720 (QRD21_22720)	pilD	4860597..4861394 (+)	798	WP_286114734.1	A24 family peptidase	Machinery gene
QRD21_RS22725 (QRD21_22725)	hrpB	4861490..4863919 (-)	2430	WP_286114735.1	ATP-dependent helicase HrpB	-
QRD21_RS22730 (QRD21_22730)	thpR	4863992..4864528 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
QRD21_RS22735 (QRD21_22735)	sfsA	4864528..4865244 (+)	717	WP_286114736.1	DNA/RNA nuclease SfsA	-
QRD21_RS22740 (QRD21_22740)	dksA	4865409..4865864 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29180.80 Da
• Isoelectric Point: 8.3358

>NTDB_id=844988 QRD21_RS22720 WP_286114734.1 4860597..4861394(+) (pilD) [Klebsiella michiganensis strain ELP1.34B]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFTPLAEAVVGAMAGYLSLWLVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=844988 QRD21_RS22720 WP_286114734.1 4860597..4861394(+) (pilD) [Klebsiella michiganensis strain ELP1.34B]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTACCCCCTGATGGAGCTGACAACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCACGCCGCTGGCGGAGGCGGTCGTTGGCGCGATGGCCGGGTATTTGTCGCTGTGGTTGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTACTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.339	100	0.494
pilD	Neisseria gonorrhoeae MS11	45.324	100	0.475
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438