Detailed information NTG0844414

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QLQ02_RS01295
• Genome accession: NZ_CP127833
• Coordinates: 220447..224151 (-)
• Length: 1234 a.a.
• NCBI ID: WP_286058758.1
• Uniprot ID: -
• Organism: Bacillus mojavensis strain KRS009
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 215447..229151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QLQ02_RS01280	hlpB	215492..215794 (-)	303	WP_229147779.1	HNH nuclease-like protein HlpB	-
QLQ02_RS01285	sbcC	215808..219200 (-)	3393	WP_286058756.1	exonuclease subunit SbcC	-
QLQ02_RS01290	sbcD	219197..220375 (-)	1179	WP_286058757.1	exonuclease subunit SbcD	-
QLQ02_RS01295	addA	220447..224151 (-)	3705	WP_286058758.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QLQ02_RS01300	addB	224138..227638 (-)	3501	WP_286058759.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QLQ02_RS01305	-	227824..228237 (+)	414	Protein_259	rubrerythrin family protein	-
QLQ02_RS01310	-	228363..228677 (+)	315	WP_010333659.1	four-helix bundle copper-binding protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141464.95 Da
• Isoelectric Point: 5.1871

>NTDB_id=844414 QLQ02_RS01295 WP_286058758.1 220447..224151(-) (addA) [Bacillus mojavensis strain KRS009]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEEHPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSPIGELPFYPYVKEDIAMVLEGAKEKLQRA
LELTKAPGGPAPRAKNFLDDLAQIDDLIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGSGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVVLLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAASDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAILRGEPVPGTFAYEEKARERLAWKYTHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVTPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTTEEAERTVRHLYEKELLTEEQQSAIDIEEIVQFFHTEIGE
KLIGAKWKDREVPFSLALPAKEIYPDAEEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=844414 QLQ02_RS01295 WP_286058758.1 220447..224151(-) (addA) [Bacillus mojavensis strain KRS009]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGATCTGGTAAAACGGCTGTTCTTGTGGAAAGAATGATCCGGAAAATCACGGCGGAAGAACATC
CGATCGATGTAGACCGGCTTCTTGTTGTCACCTTTACAAACGCCTCAGCTGCAGAGATGAAACACCGGATTGCGGATGCG
CTGGAAAAAGAGCTGGTAAAACGCCCAGGTTCGCTGCATATCCGTCGCCAGCTGTCGCTGTTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAACTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGCGATAAGGCG
TTTTTTGAGCTTGTCGACCGATACACAACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCAGATCCGGAAACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCCCGATCG
GGGAACTGCCGTTTTATCCATATGTCAAAGAAGATATTGCGATGGTGCTGGAGGGAGCAAAGGAAAAGCTTCAGCGTGCT
CTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCGCGCGCTAAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATAAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGACCCCGTCTTGCTTGATGAAGCGACAGACTTGAGAAATGGCGCCAAAAAACTCCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATTTAAAGAGTTTGGCTGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACGGCTGTGAACGAAAAGGGTGAACGTGAACCAAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTCGATGAATATCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAGCAGTCGATTTATCGATTCAGGCTAGCGGAGCCGCTCCTTT
TTCTGTCTAAGTACAAACGGTTTACAGAGAGCGGAGAAGGGTCGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAACTGAAACTCGGCGCGGCGTATCCGGAAAACAATGAAACGGAAACCGAACTGCTGCTGATTGACAAGTCCG
AGGAGACGGACGCAGGAGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTCGAGGCAAAAGCGATCGCTAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGAAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGGT
TTTGCTGCGATCCATGCCGTGGGCCCCGCAAATCATGGAAGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAAGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTTTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAATGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAGTATATGGATTATGTTGGCGGAATGCCGGGCGGCAAACAGCGGCAGGCTAACTTGCGCGTCCTCTATGACCGGGC
ACGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAAGATGTTGTCCGCTTAATGACCATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTTTTCACAGCGGGACTTGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTATCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCTGAGGAATTGCGTGTGCTCTATGTTGCTTTAACGCGGGCAAAGGAGAAGCTGTTTCTT
GTCGGCTCATGCAAGGATCACCAGAAACAGCTGGCAAAATGGCAGGCATCGGCATCCCAAGCTGACTGGCTCCTGCCGGA
ATTTGATCGCTATCAGGCGAAAACGTATTTGGATTTCATTGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGTGCGGCAGCGGCTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAGCGTCTTGAAGCGATCCTCCGCGGGGAACCGGTTCC
CGGCACGTTTGCATATGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATACCCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTGTCTGAGATTAAGAGAAGAAGAGAGTACGAGGACGAATACAGCGGACGGGCCCCCGTTACACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAGAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGACAGAAGAAGCGGAACGGACAGTGCGGCACCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCGATATTGAGGAAATTGTTCAATTTTTCCATACCGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGGACCGTGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GGAGGAGGCGGATGAGCCGCTATTGGTGCAGGGAATCATCGATTGTCTCTACGAAACAGAGGACGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGGAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAAGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCATATTCTGACTTTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	93.355	100	0.934