Detailed information NTG0843773

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QRY57_RS07340
• Genome accession: NZ_CP127376
• Coordinates: 1157140..1160865 (+)
• Length: 1241 a.a.
• NCBI ID: WP_286017085.1
• Uniprot ID: -
• Organism: Bacillus arachidis strain YX15
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1152140..1165865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRY57_RS07325 (QRY57_07330)	-	1152294..1152887 (+)	594	WP_026590502.1	TVP38/TMEM64 family protein	-
QRY57_RS07330 (QRY57_07335)	lepB	1152945..1153508 (+)	564	WP_026590501.1	signal peptidase I	-
QRY57_RS07335 (QRY57_07340)	addB	1153631..1157143 (+)	3513	WP_286017084.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QRY57_RS07340 (QRY57_07345)	addA	1157140..1160865 (+)	3726	WP_286017085.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QRY57_RS07345 (QRY57_07350)	-	1160942..1161235 (+)	294	WP_026590498.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
QRY57_RS07350 (QRY57_07355)	gerPF	1161320..1161535 (-)	216	WP_001141566.1	spore germination protein GerPF	-
QRY57_RS07355 (QRY57_07360)	-	1161581..1161967 (-)	387	WP_208017793.1	spore germination protein GerPE	-
QRY57_RS07360 (QRY57_07365)	-	1161980..1162174 (-)	195	WP_090693406.1	spore gernimation protein GerPD	-
QRY57_RS07365 (QRY57_07370)	-	1162181..1162795 (-)	615	WP_098303074.1	spore germination protein GerPC	-
QRY57_RS07370 (QRY57_07375)	-	1162879..1163070 (-)	192	WP_026590494.1	spore germination protein GerPB	-
QRY57_RS07375 (QRY57_07380)	-	1163087..1163308 (-)	222	WP_026590493.1	spore germination protein	-
QRY57_RS07380 (QRY57_07385)	-	1163479..1163658 (-)	180	WP_026590492.1	aspartyl-phosphate phosphatase Spo0E family protein	-
QRY57_RS07385 (QRY57_07390)	-	1163900..1164799 (+)	900	WP_286017086.1	fumarylacetoacetate hydrolase family protein	-
QRY57_RS07390 (QRY57_07395)	-	1164771..1165274 (+)	504	WP_231503893.1	HXXEE domain-containing protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 143137.61 Da
• Isoelectric Point: 5.1447

>NTDB_id=843773 QRY57_RS07340 WP_286017085.1 1157140..1160865(+) (addA) [Bacillus arachidis strain YX15]
MIENWPQKPEGSQWTDDQWKAVVRDGRDILVAAAAGSGKTAVLVERMIRKIINAENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDDPGSGHIRKQLSLLNKASISTIHSFCLQVIRTYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGTE
GNNIFFELVDRYTSDRSDDDLQRMILALHTESRAHPDPEKWLDKLVEAYDVEGKSIEDLTYASYLLEDVKFQLETAEKHV
QKAIELTMLPDGPAPRMETLQADLVLLGTLSSAAHQSWTSVYEAMQNVSWQTLKRIKKSDYNEEIVKQVDSLRNKAKDEV
KKLHEELFSRKPESFLRDFKEMHPVLEKLVQIVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSERTENGELRPSPVALQ
YRNKFTEVLVDEYQDTNFVQESIIKFVTKDSEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTAEGIDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGETVGEIEYDADAELKLGASYPEGEDVAAELLCIHQSEGEESAEGEDGVEVEKAQLEARL
MAGRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVTVMMNVFRVID
NPMQDIPLAAVLRSPIVGLHDEELAMLRAHEKKGSFYEVMRSFLQGAPLEEEAELHGKLQWFYNLLQGWREFARQQSLSD
LIWKVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMT
IHKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALT
RAKEKLILIGTVKDKAKEMEKWLDAREHTDWLLPDYLRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPNEIYEYDASW
KVEIVDGSTLLAPEPVQEEKQELLEALREKKTVPLESERKEEVYARLTWEYAHEGATMHRAKQSVTEIKRNYQSEDGSDH
AFIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVNLQKTITPETIKEQIAEMVNKELLTFEQAEEIAIDRIVAFFD
TDLGKRVLAAKSVEREVPFTMMLSAKEAYQEWEGQSKESILVQGVIDCMIEEEDGIVLIDFKTDTIEGRFPGGYDQARPI
LEDRYKVQINLYAKALEKSLHHPVKGKYLYFFDGNHIVHVD

Nucleotide

• Length: 3726 bp

>NTDB_id=843773 QRY57_RS07340 WP_286017085.1 1157140..1160865(+) (addA) [Bacillus arachidis strain YX15]
ATGATAGAGAATTGGCCACAGAAACCTGAAGGTAGTCAATGGACAGATGATCAATGGAAGGCAGTTGTCAGAGATGGCCG
TGATATTTTAGTCGCGGCGGCAGCTGGGTCAGGAAAGACAGCTGTACTTGTAGAAAGAATGATTCGCAAAATTATTAATG
CTGAGAATCCAGTTGATGTAGATCGTCTGCTTGTCGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAGAACAGAATT
GGCGAAGCGTTAGAAAAAGTATTAATTGATGATCCAGGGTCAGGGCATATTCGTAAGCAGCTCAGCTTATTGAATAAAGC
ATCGATTTCAACGATTCACTCGTTCTGTTTACAAGTTATTCGAACGTACTACTATATGCTCGACGTTGATCCGCGTTTTC
GCATTGCAAACCAGACAGAAAATGAATTATTAAAAGAAGAAGTATTAGATGACATATTAGAAGAGGAATATGGCACAGAG
GGAAATAATATTTTCTTTGAGCTTGTTGATCGCTATACGAGTGACCGAAGTGATGATGACTTGCAGCGCATGATTTTAGC
GCTTCATACAGAATCAAGAGCACATCCAGATCCAGAGAAATGGCTTGATAAGCTAGTGGAAGCATATGATGTGGAAGGAA
AATCAATTGAGGATTTAACGTATGCTTCTTACTTATTAGAAGATGTGAAGTTTCAGCTAGAAACTGCCGAAAAGCATGTT
CAAAAAGCAATTGAACTTACTATGCTTCCAGATGGGCCAGCACCCCGCATGGAGACATTACAAGCAGATTTGGTGTTACT
CGGAACGTTATCTAGTGCAGCGCATCAGTCTTGGACAAGTGTCTATGAAGCGATGCAAAATGTTTCGTGGCAAACGCTAA
AGCGTATTAAGAAGAGTGACTACAATGAAGAGATTGTAAAACAAGTAGATTCACTTCGAAATAAAGCAAAAGATGAAGTG
AAGAAGTTACATGAAGAGTTGTTTAGCCGAAAACCAGAAAGTTTTTTACGTGATTTTAAGGAAATGCATCCTGTACTAGA
AAAGCTTGTTCAGATTGTAAAAGTATTTACGGAGCGATTCCAAGCGATCAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTTTGCTTACAAATTTTAAGTGAACGTACAGAAAATGGTGAGCTTCGTCCGTCACCGGTTGCGCTTCAA
TATCGCAATAAATTTACTGAAGTACTTGTCGACGAATATCAAGATACAAACTTTGTCCAGGAGTCGATTATTAAGTTTGT
AACGAAAGACTCTGAGCAAGAAGGTAATCTGTTTATGGTTGGCGACGTAAAACAATCGATTTATCGATTCCGACTAGCAG
AACCGGGCCTTTTCCTAGGGAAATATAAACGTTTTACGGCTGAAGGAATAGACGGCGGTATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCACGAAGTGTTAGCCGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAACGGTCGGGGAAAT
TGAGTATGACGCCGATGCTGAACTAAAGTTAGGTGCAAGTTATCCAGAAGGGGAAGATGTAGCGGCTGAGCTATTATGTA
TTCATCAGTCTGAGGGTGAAGAATCGGCAGAGGGAGAAGACGGTGTTGAAGTAGAAAAAGCACAGCTGGAAGCGCGTTTA
ATGGCCGGGAGAATTAAAGCGATGGTGGATTCCGGTTATGAAGTATACGATCGTAAAACAGATAGCATGCGCCCGGTGAA
ATATCGTGACTTTGTTATTTTACTTCGCTCGATGCCGTGGGCTCCTCAAATTATGGAAGAATTAAAGTTGCAAGGTATTC
CTGTTTACGCTGAGCTTGCAACGGGTTATTTTGAAGCAACAGAAGTGACCGTTATGATGAATGTATTTCGCGTAATCGAT
AATCCGATGCAAGACATTCCGCTTGCCGCTGTACTACGTTCCCCAATTGTTGGATTACATGATGAGGAGCTTGCGATGCT
TCGTGCTCATGAAAAGAAAGGTTCGTTTTATGAAGTGATGCGTTCCTTCTTACAAGGAGCGCCGCTTGAGGAAGAGGCGG
AATTACACGGAAAACTACAATGGTTCTATAACCTTCTGCAAGGATGGCGTGAATTTGCACGCCAGCAATCACTTTCTGAT
TTGATTTGGAAAGTATATCGTGAGACAGGTTATTATGACTTTGTTGGTGGTCTTCCAGCAGGAAAACAGCGCCAGGCTAA
CTTACGCGTATTATACGACCGGGCAAGGCAGTATGAAGCAACATCTTTCCGCGGGTTATTCCGTTTCCTTCGCTTTATTG
AGCGGATTTTAGAGCGCGGAGATGATATGGGAACAGCGAGAGCGCTTGGCGAGCAAGAAGATGTTGTGCGAATTATGACG
ATTCATAAAAGTAAGGGGTTAGAGTTCCCGGTTGTATTTGTAGCGGGACTTGGACGCCGTTTTAATACACAAGATTTAAT
GAAACGATTCTTATTACACAAGGATTTCGGTTTTGGATCACAGTTTATTGATCCTCGTAAGCGAATTAAATATACGACGC
TATCGCAATTGGCCATTAAACGTAAAATGAAGATGGAACTTATTGCGGAAGAGATGCGCGTATTATATGTTGCTTTAACG
CGTGCGAAAGAGAAGTTAATTTTAATTGGTACAGTGAAAGATAAAGCAAAAGAAATGGAAAAGTGGCTTGATGCAAGAGA
GCATACAGATTGGTTATTACCAGATTATCTTCGTGCGGGTGCATCTTGTTACTTAGATTGGATTGCGCCGTCACTATATA
GACATCGTGATAGTGAAATGCTTCTTGAACTTGGACAAGGAAATATACCAAATGAAATTTATGAATACGATGCGAGCTGG
AAAGTGGAAATCGTTGATGGTAGCACTTTACTTGCACCTGAACCAGTACAAGAAGAGAAGCAAGAATTGTTAGAAGCACT
TCGTGAGAAAAAAACTGTTCCGCTAGAAAGTGAACGAAAAGAAGAAGTGTATGCTCGTTTAACATGGGAATATGCGCATG
AAGGTGCAACAATGCACCGGGCGAAGCAATCTGTTACGGAAATAAAGCGGAATTATCAGTCAGAAGATGGAAGTGATCAT
GCTTTTATTAAAAAACTTCGTGCTCCTATAAAAACGCGACCTCGTTTTATGGAGAAAAAGGGGTTGACCTACGCTGAACG
AGGGACAGCTGTCCACGCCGTCATGCAGCATGTTAATTTACAAAAAACGATTACACCAGAAACAATCAAAGAACAAATTG
CGGAAATGGTAAATAAAGAATTACTAACATTTGAGCAAGCAGAAGAAATTGCGATTGATCGAATTGTAGCATTTTTTGAT
ACGGATCTTGGTAAACGTGTGTTAGCTGCGAAGAGTGTAGAGCGTGAAGTGCCCTTTACGATGATGCTTTCAGCAAAAGA
GGCATATCAAGAGTGGGAAGGTCAGAGCAAAGAATCTATACTTGTCCAAGGGGTTATTGATTGTATGATAGAAGAGGAAG
ATGGGATTGTATTAATTGATTTTAAAACAGATACAATTGAAGGGCGTTTTCCGGGTGGATACGATCAAGCGCGACCTATT
TTAGAAGACCGTTATAAAGTGCAAATTAATCTATATGCGAAAGCGCTAGAGAAAAGCTTACATCATCCTGTGAAGGGGAA
ATATTTATACTTCTTTGATGGGAATCACATAGTACATGTTGATTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1061-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-891)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	54.05	100	0.543