Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QRY57_RS07335 Genome accession   NZ_CP127376
Coordinates   1153631..1157143 (+) Length   1170 a.a.
NCBI ID   WP_286017084.1    Uniprot ID   -
Organism   Bacillus arachidis strain YX15     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1148631..1162143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRY57_RS07310 (QRY57_07315) aceA 1148697..1149974 (+) 1278 WP_286017083.1 isocitrate lyase -
  QRY57_RS07315 (QRY57_07320) - 1150315..1150518 (+) 204 WP_003195857.1 cold-shock protein -
  QRY57_RS07320 (QRY57_07325) - 1151482..1152069 (+) 588 WP_090693418.1 competence protein ComK -
  QRY57_RS07325 (QRY57_07330) - 1152294..1152887 (+) 594 WP_026590502.1 TVP38/TMEM64 family protein -
  QRY57_RS07330 (QRY57_07335) lepB 1152945..1153508 (+) 564 WP_026590501.1 signal peptidase I -
  QRY57_RS07335 (QRY57_07340) addB 1153631..1157143 (+) 3513 WP_286017084.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QRY57_RS07340 (QRY57_07345) addA 1157140..1160865 (+) 3726 WP_286017085.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QRY57_RS07345 (QRY57_07350) - 1160942..1161235 (+) 294 WP_026590498.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  QRY57_RS07350 (QRY57_07355) gerPF 1161320..1161535 (-) 216 WP_001141566.1 spore germination protein GerPF -
  QRY57_RS07355 (QRY57_07360) - 1161581..1161967 (-) 387 WP_208017793.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134005.94 Da        Isoelectric Point: 5.0826

>NTDB_id=843772 QRY57_RS07335 WP_286017084.1 1153631..1157143(+) (addB) [Bacillus arachidis strain YX15]
MSLRFVIGRAGSGKSTFCLHEVQEELRQRPRGETILYLVPEQMTFQTQQALIGNEEVSGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKEGLYVFQKAAEQNGFFEHLGSMISEFKRYNVTPSNVYEMWQQLDTHSIGDEQKLLA
NKMYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPGSDYVKGAEIYIDGFHSFSPGELEIIRRLMLCGARITVTLTVDE
KTLAQPVSELDLFYQTVLTYEKVKQLAREEKIAIENTISLREQPRFRSPALAHLEAEFESRPAKKFQGEPDITLYTAANL
RAEVEGVAREVRRLVAEEEYRYRDIAVLLRNGESYYDVMKTLFTDYDIPHFIDEKRPMSHHPLIECIRSALEIISGNWRY
DAIFRCIKTELLYPLDVRKESMREEMDAFENYCLAYGVQGKRWTADEPWTYRRYRSLDGAFGAQTDSEREMEEKINRLRD
VVRTPIIRLQKRLKRSGSIVQMCEAVYLFLEELDVPKKLEDLRMRAEAEGNFLFASDHDQVWEEVMNLLDTFVEMLGEEK
MSLSMFIDVMTTGLEALQFANIPPSLDQVLIANIDRSRLSNVRATFVIGANEGIIPAAPSDEGMLSDEERELLTTAGVEL
APTTRQTLLEEQFVIYQTVTRASEKLYISCPLADEEGKTLLSSSFIKKVKRMFPSIKEVFVTNDVNDLSRQAQISYVATP
EVTLSYVTQQLQNWKRYGFEGNLDFWWDVYNFYVTSPEWEQKSSRVLSSLFYRNRAQKLSPVVSRNLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSAAVIEEIAPLLQRQIL
LSSNRNHYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFTLANGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSTKALDLTEVYYGLALQMLTYLDVVISNANTWMKKGQAASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFTTLQQYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFRSVCQFDESLEDNEFRTLKDMKDSEAMEKMREEVGE

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=843772 QRY57_RS07335 WP_286017084.1 1153631..1157143(+) (addB) [Bacillus arachidis strain YX15]
ATGTCGCTACGATTTGTGATTGGACGGGCTGGAAGTGGAAAAAGTACATTTTGTTTACACGAAGTACAAGAAGAATTAAG
ACAGCGTCCAAGAGGCGAAACCATTTTATATCTTGTACCGGAACAAATGACGTTCCAAACGCAGCAAGCATTAATAGGAA
ATGAAGAAGTTAGCGGCTCGATCAGGGCGCAAGTGTTTAGTTTTTCAAGGTTAGCATGGAAGGTTCTGCAAGAAGTTGGT
GGCGCAAGCCGTCTTCATATTGATGAGGCAGGCGTACATATGCTACTGCGCAAAATTGTAGAATCGCGTAAGGAAGGATT
ATATGTGTTTCAAAAAGCAGCAGAGCAAAATGGCTTTTTTGAGCACTTAGGTAGTATGATTTCCGAGTTTAAACGCTATA
ATGTAACGCCATCGAATGTATATGAGATGTGGCAACAATTAGATACTCATTCAATTGGTGATGAACAAAAATTATTAGCA
AATAAAATGTACGATCTTCAGCTTTTATATGATGATTTTGAACGGGCTTTAATTGGTAAATATTTAGATTCAGAAGATTA
TTTACAGTTACTTGTAGAAAAGCTTCCTGGTTCGGACTATGTAAAAGGTGCAGAAATTTATATAGATGGGTTCCATTCCT
TTTCACCTGGGGAATTAGAAATTATTAGAAGGCTTATGCTTTGCGGAGCAAGAATTACAGTTACGTTAACAGTAGATGAA
AAAACGTTAGCACAGCCAGTAAGTGAATTAGATTTGTTTTATCAAACCGTGCTTACGTATGAAAAAGTGAAACAGTTAGC
ACGTGAGGAGAAAATTGCAATTGAAAATACGATTTCGCTGAGAGAACAGCCGCGTTTTCGCTCACCAGCACTAGCACATT
TAGAAGCTGAGTTTGAATCTCGTCCTGCTAAGAAGTTTCAAGGTGAACCAGATATTACATTATATACAGCGGCAAACTTG
CGAGCAGAAGTTGAAGGTGTAGCTAGAGAAGTTCGCAGACTTGTTGCGGAAGAGGAGTATCGCTACAGAGATATTGCGGT
TCTTCTTCGTAACGGAGAAAGCTATTATGATGTAATGAAAACATTGTTTACGGATTATGATATTCCGCATTTTATCGATG
AGAAGCGCCCGATGTCACATCATCCATTAATTGAATGTATTCGTTCTGCGCTAGAAATTATTAGCGGAAATTGGCGTTAT
GATGCGATATTCCGCTGTATAAAAACGGAACTGTTATATCCGTTAGATGTAAGAAAAGAATCAATGCGTGAAGAAATGGA
CGCGTTTGAAAATTACTGTTTAGCATATGGCGTACAAGGAAAGAGATGGACGGCAGATGAACCGTGGACATATCGCCGTT
ATCGTTCGTTAGATGGGGCATTTGGAGCGCAAACAGATAGTGAACGTGAAATGGAAGAGAAAATAAACCGCCTTCGTGAT
GTGGTACGAACACCGATTATTCGTTTGCAGAAACGCTTAAAACGTTCCGGTTCAATTGTACAGATGTGTGAAGCGGTATA
TTTGTTTTTAGAAGAATTAGATGTTCCTAAAAAACTAGAAGATTTGCGTATGCGTGCTGAGGCAGAGGGGAATTTCTTAT
TTGCAAGTGACCATGATCAAGTTTGGGAAGAAGTCATGAACCTTCTTGATACATTTGTAGAGATGCTTGGTGAAGAAAAG
ATGTCTCTTTCGATGTTTATCGATGTAATGACAACTGGGCTTGAGGCACTTCAATTTGCAAATATCCCGCCTTCTTTAGA
CCAAGTGCTTATTGCAAATATTGATCGATCAAGGTTATCGAATGTTCGTGCAACTTTTGTAATTGGTGCGAATGAAGGAA
TCATTCCAGCAGCCCCTTCAGATGAAGGCATGTTATCAGATGAAGAAAGAGAGCTTCTCACCACAGCTGGTGTAGAATTA
GCGCCGACAACAAGGCAGACTTTATTAGAAGAGCAATTTGTGATTTATCAAACCGTAACTCGTGCTTCTGAGAAGCTATA
CATTTCTTGTCCACTCGCGGATGAAGAAGGAAAAACGTTACTTTCATCTAGCTTTATTAAGAAAGTGAAAAGAATGTTCC
CTTCTATTAAAGAAGTATTTGTAACGAATGATGTGAATGACTTGTCTCGTCAGGCGCAAATTTCGTATGTAGCAACGCCA
GAAGTTACCTTGTCATATGTAACGCAACAATTACAAAACTGGAAACGATACGGTTTTGAAGGAAATCTTGATTTTTGGTG
GGATGTATATAACTTCTATGTTACATCGCCCGAGTGGGAGCAAAAAAGTAGTCGGGTATTATCTAGTTTATTTTATCGAA
ACCGTGCACAGAAACTCAGCCCAGTTGTAAGTAGAAATCTATACGGAGATACAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGCTGCGCGTATGCTCATTTTGCACAGCATGGATTATCACTAAGAGAGCGTGATATTTTCAAATTAGATGC
ACCGGATATCGGTGAATTATTCCATGCGGCACTAAAGAAAATTGCAGATAAGTTACTTAGGGAAAATCGCACTTGGGCTG
ATTTATCGATTAAAGAGTGTGAGCATTTGTCTGCGGCAGTAATTGAAGAAATCGCACCGCTTCTGCAAAGGCAAATTTTA
TTAAGTTCAAATCGGAATCATTATTTAAAACAAAAACTGCAGCAAATTATTTTCCGTACGTCACTTATCCTTCGTGAACA
TGCGAAGTCTAGTGGATTCGTGCCAGTTGACTTAGAGGTTCCATTCGGTATGGGCGGGACAGGTTCACTTCCGCCGATGG
AGTTTACACTCGCAAACGGTGTGAAAATGGAAGTTGTTGGCCGGATTGACCGCGTAGATAAAGCGGAAGATGAAAACGGT
ACGTTCCTGCGGATTATTGATTATAAATCAAGCACGAAAGCGTTAGATTTAACAGAAGTATATTATGGTTTAGCACTTCA
AATGTTAACGTATTTAGACGTTGTCATTTCAAATGCAAATACGTGGATGAAAAAGGGACAGGCGGCATCACCTGCTGGTG
TTCTATATTTCCATATTCATAACCCGATTGTGGAAATGAAAGGCGATGCTTCGGAAGAAGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTGTTAGGAGATGCGGATGTTGTTCGATTGATGGACAATAAGTTAAGTACGGGAAG
TTCAGACATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGCGCGCGTTCTAGCATTGCAAGTGAACAAGAATTTA
CAACTTTACAGCAATATGTGCATCATACATTTGAAAACATCGGGAAAGACATTACAGAAGGTGTGATTGATATTGCACCG
TATAAAATGGGGAATAAGGCTGCTTGTACGTTCTGTAATTTCCGCTCTGTTTGTCAGTTTGATGAATCACTTGAAGATAA
CGAGTTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATGAGAGAGGAGGTTGGCGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.06

100

0.491