Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   QRA12_RS03635 Genome accession   NZ_CP127345
Coordinates   739134..740762 (-) Length   542 a.a.
NCBI ID   WP_001186604.1    Uniprot ID   Q8VUV3
Organism   Staphylococcus aureus strain MRSA11     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 718272..747685 739134..740762 within 0


Gene organization within MGE regions


Location: 718272..747685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRA12_RS03525 (QRA12_03520) ccrC 718272..719951 (+) 1680 WP_016187646.1 cassette chromosome recombinase CcrC -
  QRA12_RS03530 (QRA12_03525) - 720040..720378 (+) 339 WP_031796882.1 SAUGI family uracil-DNA glycosylase inhibitor -
  QRA12_RS03535 (QRA12_03530) - 720472..720783 (+) 312 WP_168754273.1 DUF960 family protein -
  QRA12_RS03540 (QRA12_03535) - 720801..721304 (+) 504 WP_168754274.1 DUF1643 domain-containing protein -
  QRA12_RS03545 (QRA12_03540) - 721318..721539 (+) 222 WP_168754275.1 hypothetical protein -
  QRA12_RS03550 (QRA12_03545) - 721618..723885 (-) 2268 WP_168754276.1 CDP-glycerol:glycerophosphate glycerophosphotransferase -
  QRA12_RS03555 (QRA12_03550) - 724142..724324 (+) 183 WP_031864945.1 hypothetical protein -
  QRA12_RS03560 (QRA12_03555) - 725366..725666 (-) 301 Protein_695 type I restriction-modification system subunit M N-terminal domain-containing protein -
  QRA12_RS03565 (QRA12_03560) - 725848..726717 (+) 870 WP_168754285.1 hypothetical protein -
  QRA12_RS03570 (QRA12_03565) - 727138..728393 (+) 1256 Protein_697 MrcB family domain-containing protein -
  QRA12_RS03575 (QRA12_03570) - 728808..729047 (+) 240 WP_001282728.1 hypothetical protein -
  QRA12_RS03580 (QRA12_03575) - 729079..729753 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  QRA12_RS03585 (QRA12_03580) - 729789..730178 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  QRA12_RS03590 (QRA12_03585) - 731055..731798 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  QRA12_RS03595 (QRA12_03590) - 731895..732323 (+) 429 WP_000872606.1 MaoC family dehydratase -
  QRA12_RS03600 (QRA12_03595) mecA 732369..734378 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  QRA12_RS03605 (QRA12_03600) - 734475..735461 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  QRA12_RS03610 (QRA12_03605) - 735451..735693 (+) 243 Protein_705 type I restriction enzyme endonuclease domain-containing protein -
  QRA12_RS03615 (QRA12_03610) - 735684..737294 (-) 1611 Protein_706 IS1182 family transposase -
  QRA12_RS03620 (QRA12_03615) - 737343..737849 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  QRA12_RS03625 (QRA12_03620) - 737864..738175 (-) 312 WP_000700851.1 DUF960 family protein -
  QRA12_RS03630 (QRA12_03625) - 738262..738612 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  QRA12_RS03635 (QRA12_03630) ccrB 739134..740762 (-) 1629 WP_001186604.1 cassette chromosome recombinase CcrB Machinery gene
  QRA12_RS03640 (QRA12_03635) ccrA 740784..742133 (-) 1350 WP_000815652.1 cassette chromosome recombinase CcrA Machinery gene
  QRA12_RS03645 (QRA12_03640) cch1 742367..744154 (-) 1788 WP_000122621.1 cassette chromosome replicative helicase -
  QRA12_RS03650 (QRA12_03645) ssb 744154..744444 (-) 291 WP_406707337.1 cassette chromosome ssDNA-binding protein -
  QRA12_RS03655 (QRA12_03650) - 744583..745632 (+) 1050 WP_000613248.1 hypothetical protein -
  QRA12_RS03660 (QRA12_03655) - 746195..747685 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62650.85 Da        Isoelectric Point: 9.9805

>NTDB_id=843631 QRA12_RS03635 WP_001186604.1 739134..740762(-) (ccrB) [Staphylococcus aureus strain MRSA11]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
IYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVARVNQENKVDGAALHHDIAYKQQQYD
EVQTKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKSLFDAKQISKLLQHIFQDIKHMEKSRLK
ALYLSVIDRINIRKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=843631 QRA12_RS03635 WP_001186604.1 739134..740762(-) (ccrB) [Staphylococcus aureus strain MRSA11]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGATCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATAGATTGTGTTATG
ATCTACAAAACAAACCGATTAGCTCGTAATACATCGGATCTTCTGAAAATCGTCGAAGATTTGCATAAACAAAATGTCGA
ATTTTTCAGTTTATCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGGCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTGAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTTCATGGTAAAGGAACGA
ATCTGCTTACAGGCATTATCCATTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACAACGAATACACTTAAAGAT
GGGACCAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTAATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTTG
TAGCACGTGTTAATCAAGAAAATAAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAACCAAACTAAATAACTTGATTAAAACCATAGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGTCTT
TATTTGATGCCAAACAAATCAGTAAACTATTACAACACATCTTTCAAGATATTAAGCACATGGAAAAATCTCGACTCAAA
GCATTGTACCTATCAGTGATTGATCGTATTAATATTAGAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

97.417

100

0.974