Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VR615_RS22040 Genome accession   NZ_CP142843
Coordinates   4512746..4513543 (-) Length   265 a.a.
NCBI ID   WP_032347795.1    Uniprot ID   -
Organism   Escherichia coli strain SX45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4507746..4518543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VR615_RS22020 (VR615_22080) gspD 4508414..4510474 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  VR615_RS22025 (VR615_22085) gspC 4510504..4511463 (-) 960 WP_000135076.1 type II secretion system protein GspC -
  VR615_RS22030 (VR615_22090) gspS2 4511481..4511891 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  VR615_RS22035 (VR615_22095) - 4512038..4512735 (+) 698 WP_103215986.1 IS1-like element IS1A family transposase -
  VR615_RS22040 (VR615_22100) pilD 4512746..4513543 (-) 798 WP_032347795.1 prepilin peptidase PppA Machinery gene
  VR615_RS22045 (VR615_22105) sslE 4513741..4518303 (-) 4563 WP_025693395.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29049.54 Da        Isoelectric Point: 8.5960

>NTDB_id=842279 VR615_RS22040 WP_032347795.1 4512746..4513543(-) (pilD) [Escherichia coli strain SX45]
MLFDVFQQYPTAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRIRDNIP
LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYR

Nucleotide


Download         Length: 798 bp        

>NTDB_id=842279 VR615_RS22040 WP_032347795.1 4512746..4513543(-) (pilD) [Escherichia coli strain SX45]
ATGCTTTTTGATGTTTTTCAGCAATACCCCACGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCAATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGTTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTTGCTTTTTTGCTGGCGAGTCTGGTCTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATCGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGCTCACGCTACAAGATGCAGTCACCGGCGTCCTGGTGGGATTTATCAC
TTTTTACTCTCTGCGTTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCCG
CGTTAGGTGGCTGGGTGGGGGCATTGTCGCTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCGGTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.944

100

0.453
  pilD Vibrio campbellii strain DS40M4

42.435

100

0.434
  pilD Neisseria gonorrhoeae MS11

39.3

96.981

0.381
  pilD Acinetobacter nosocomialis M2

38.113

100

0.381
  pilD Acinetobacter baumannii D1279779

37.736

100

0.377