Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   QMC72_RS19110 Genome accession   NZ_CP127095
Coordinates   3775213..3778917 (-) Length   1234 a.a.
NCBI ID   WP_284106723.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain LBA001     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3770213..3783917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMC72_RS19095 (QMC72_19095) hlpB 3770261..3770563 (-) 303 WP_044427342.1 HNH nuclease-like protein HlpB -
  QMC72_RS19100 (QMC72_19100) sbcC 3770577..3773969 (-) 3393 WP_284106721.1 exonuclease subunit SbcC -
  QMC72_RS19105 (QMC72_19105) sbcD 3773966..3775141 (-) 1176 WP_284106722.1 exonuclease subunit SbcD -
  QMC72_RS19110 (QMC72_19110) addA 3775213..3778917 (-) 3705 WP_284106723.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QMC72_RS19115 (QMC72_19115) addB 3778904..3782404 (-) 3501 WP_284106724.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QMC72_RS19120 (QMC72_19120) - 3782587..3783024 (+) 438 WP_044427329.1 ferritin family protein -
  QMC72_RS19125 (QMC72_19125) - 3783143..3783469 (+) 327 WP_044427326.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 140967.25 Da        Isoelectric Point: 5.1228

>NTDB_id=841712 QMC72_RS19110 WP_284106723.1 3775213..3778917(-) (addA) [Bacillus inaquosorum strain LBA001]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQGDFTELYKRVPAVSFKRAKTVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQATASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAENGAGAHADISGHPARFAVQIIHSYD
LLDDDLEERLEEKSERLEAIRRGEPVPGPFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPGKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
GLIGAKWKDREVPFSLALPAKEIYPGAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=841712 QMC72_RS19110 WP_284106723.1 3775213..3778917(-) (addA) [Bacillus inaquosorum strain LBA001]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATTACCGCAGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAACGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGTATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTAAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTACTTGGGGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCGAAGGGTGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTATGAATA
TTCCCGTTCTCATCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGCGCAATCG
AGGAGCTTCCATTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTGCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGGCGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAACAGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCATTTGAAAAGCTTAGCCGACATGAAGCCTGTTATTGAAACACTCGTACA
GCTTGTCATTAGCTACGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCAGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGACAAAGGCGAACGAGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAAGAGACCGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCAGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGACGGAAAATCGACCTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTACCCGGACAATGATGAGACGGAAACAGAGCTGCTGCTGATCGACAACTCAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGT
AAGTTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
ATTGTTACGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCAGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAT
ACAAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTTGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGACGGAACTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTATCCGACGCTTCCGCTCATTGCCATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGCGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCAACTGCGTCCCAAACTGACTGGCTTCTCCCGGA
ATTTGACCGCTATCAGGCAAAAACCTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATTTGGAGGCTTTGG
CTGAAAACGGTGCGGGAGCGCATGCTGACATATCAGGCCACCCGGCTCGTTTTGCCGTTCAAATAATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGATTGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCC
CGGCCCGTTTGCGTTTGATGAGAAAGCCCGGGAGCAGCTAAGCTGGACATATCCGCATCATGAGGTGACGCAAATTCGCA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGGTAAACCGGCA
GATGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGTTTGACAGCGGCGGAAAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTCACCCATGTGCCGACAATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTTTTCCATACAGAAATCGGCGGA
GGGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCTAAGGAGATCTATCCTGGTGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATCTTGAAAAAACGATACGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACACTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.759

100

0.968