Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QMC72_RS19115 Genome accession   NZ_CP127095
Coordinates   3778904..3782404 (-) Length   1166 a.a.
NCBI ID   WP_284106724.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain LBA001     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3773904..3787404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMC72_RS19105 (QMC72_19105) sbcD 3773966..3775141 (-) 1176 WP_284106722.1 exonuclease subunit SbcD -
  QMC72_RS19110 (QMC72_19110) addA 3775213..3778917 (-) 3705 WP_284106723.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QMC72_RS19115 (QMC72_19115) addB 3778904..3782404 (-) 3501 WP_284106724.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QMC72_RS19120 (QMC72_19120) - 3782587..3783024 (+) 438 WP_044427329.1 ferritin family protein -
  QMC72_RS19125 (QMC72_19125) - 3783143..3783469 (+) 327 WP_044427326.1 four-helix bundle copper-binding protein -
  QMC72_RS19130 (QMC72_19130) - 3783507..3784160 (-) 654 Protein_3777 SgrR family transcriptional regulator -
  QMC72_RS19135 (QMC72_19135) - 3784414..3785202 (+) 789 WP_232920202.1 GIY-YIG nuclease family protein -
  QMC72_RS19140 (QMC72_19140) - 3785375..3785836 (+) 462 WP_133488013.1 hypothetical protein -
  QMC72_RS19145 (QMC72_19145) - 3785836..3786243 (+) 408 WP_232920203.1 ribonuclease H -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134644.70 Da        Isoelectric Point: 5.5794

>NTDB_id=841713 QMC72_RS19115 WP_284106724.1 3778904..3782404(-) (addB) [Bacillus inaquosorum strain LBA001]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERILSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHVPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELGGTERHTRTPELAHLEAQYEARPAVPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKLREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDMSDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRTLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=841713 QMC72_RS19115 WP_284106724.1 3778904..3782404(-) (addB) [Bacillus inaquosorum strain LBA001]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGCATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGACATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTATTTTATCTGAG
AAGCTTCATGATTTATCGATTTTATATCAACAGATGGAAAAAAGCCTTGCAGGTCAATACCTTCACTCTGAGGATTATTT
AACATTGCTGGCAGAGCATGTTCCGCTTGCAGAAGATATAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCACCAAAAGGCGAA
GGAACTGAATCTGGACATTACGTATAAAGAGTTAGGCGGGACTGAACGGCATACGCGCACACCGGAATTGGCGCATCTAG
AGGCGCAGTATGAAGCGCGTCCAGCCGTTCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCAATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCACTTAACGAGCCGAAGACTAAGTTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCTCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCAGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGTACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCAGATGAAAACGGGGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTGTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTCTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTCAATAAAAGT
GTGGCACAGTCCTTTACCGCCAGCCAGCTCAGGTTATGGACTCGGGAATACGACATGAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTATTCTCAAGCCTTTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTCTCCTATGATGCGGTAGAGCGTCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAAGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACTTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAACCTCCCGCTTGGGCTTGACGAGATCGAACAGGAGATCTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATTATCAACGCCGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCTGCAGTCGGCGAAAAGGAATTTGAGCTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCACGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(306-615)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.856

100

0.979