Detailed information NTG0841327

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: QQO10_RS08475
• Genome accession: NZ_CP127021
• Coordinates: 1715111..1715608 (-)
• Length: 165 a.a.
• NCBI ID: WP_001803317.1
• Uniprot ID: A0A2I7Y542
• Organism: Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-021_7037M
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1710111..1720608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QQO10_RS08450 (QQO10_08450)	gcvPB	1710273..1711745 (-)	1473	WP_000202189.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
QQO10_RS08455 (QQO10_08455)	gcvPA	1711738..1713084 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
QQO10_RS08460 (QQO10_08460)	gcvT	1713104..1714195 (-)	1092	WP_000093351.1	glycine cleavage system aminomethyltransferase GcvT	-
QQO10_RS08465 (QQO10_08465)	-	1714354..1714878 (-)	525	WP_001015117.1	shikimate kinase	-
QQO10_RS08470 (QQO10_08470)	-	1714868..1715014 (-)	147	WP_001789879.1	hypothetical protein	-
QQO10_RS08475 (QQO10_08475)	comGF	1715111..1715608 (-)	498	WP_001803317.1	competence type IV pilus minor pilin ComGF	Machinery gene
QQO10_RS08480 (QQO10_08480)	comGE	1715526..1715825 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
QQO10_RS08485 (QQO10_08485)	comGD	1715812..1716258 (-)	447	WP_001790850.1	competence type IV pilus minor pilin ComGD	Machinery gene
QQO10_RS08490 (QQO10_08490)	comGC	1716236..1716547 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
QQO10_RS08495 (QQO10_08495)	comGB	1716561..1717631 (-)	1071	WP_000775706.1	competence type IV pilus assembly protein ComGB	Machinery gene
QQO10_RS08500 (QQO10_08500)	comGA	1717603..1718577 (-)	975	WP_000697228.1	competence type IV pilus ATPase ComGA	Machinery gene
QQO10_RS08505 (QQO10_08505)	-	1718629..1719252 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
QQO10_RS08510 (QQO10_08510)	-	1719249..1719578 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
QQO10_RS08515 (QQO10_08515)	-	1719578..1720564 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19212.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=841327 QQO10_RS08475 WP_001803317.1 1715111..1715608(-) (comGF) [Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-021_7037M]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=841327 QQO10_RS08475 WP_001803317.1 1715111..1715608(-) (comGF) [Staphylococcus aureus strain CC239-MRSA-III(var.) isolate Trinidad&Tobago_2020-021_7037M]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (67-155)

3D structure

• AlphaFold DB: A0A2I7Y542

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus MW2	99.394	100	0.994
comGF	Staphylococcus aureus N315	95.758	100	0.958