Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VLB48_RS01220 Genome accession   NZ_CP141863
Coordinates   266591..267355 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain numer 3     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 261591..272355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLB48_RS01195 yhhJ 263433..264557 (+) 1125 WP_001615305.1 ABC transporter permease -
  VLB48_RS01200 - 264630..264905 (+) 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin -
  VLB48_RS01205 - 264902..265261 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  VLB48_RS01210 nikR 265381..265782 (-) 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
  VLB48_RS01215 nikE 265788..266594 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  VLB48_RS01220 amiE 266591..267355 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  VLB48_RS01225 nikC 267355..268188 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VLB48_RS01230 nikB 268185..269129 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VLB48_RS01235 nikA 269129..270703 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  VLB48_RS01240 acpT 270814..271401 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=838583 VLB48_RS01220 WP_001136236.1 266591..267355(-) (amiE) [Escherichia coli strain numer 3]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=838583 VLB48_RS01220 WP_001136236.1 266591..267355(-) (amiE) [Escherichia coli strain numer 3]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398