Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   QN218_RS06245 Genome accession   NZ_CP126534
Coordinates   1194654..1195691 (+) Length   345 a.a.
NCBI ID   WP_060399019.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain BSY82     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1189654..1200691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN218_RS06220 - 1190492..1191820 (-) 1329 WP_060399023.1 hemolysin family protein -
  QN218_RS06225 corA 1191964..1192908 (+) 945 WP_003236968.1 magnesium transporter CorA -
  QN218_RS06230 - 1192971..1193381 (+) 411 WP_060399022.1 CBS domain-containing protein -
  QN218_RS06235 - 1193359..1193634 (+) 276 WP_060399021.1 hypothetical protein -
  QN218_RS06240 comGA 1193597..1194667 (+) 1071 WP_060399020.1 competence protein ComGA Machinery gene
  QN218_RS06245 comGB 1194654..1195691 (+) 1038 WP_060399019.1 competence type IV pilus assembly protein ComGB Machinery gene
  QN218_RS06250 comGC 1195705..1196001 (+) 297 WP_003236957.1 comG operon protein ComGC Machinery gene
  QN218_RS06255 comGD 1195991..1196422 (+) 432 WP_060399018.1 competence type IV pilus minor pilin ComGD Machinery gene
  QN218_RS06260 comGE 1196406..1196750 (+) 345 WP_060399017.1 competence type IV pilus minor pilin ComGE Machinery gene
  QN218_RS06265 comGF 1196776..1197159 (+) 384 WP_060399016.1 competence type IV pilus minor pilin ComGF Machinery gene
  QN218_RS06270 comGG 1197160..1197534 (+) 375 WP_060399015.1 competence type IV pilus minor pilin ComGG Machinery gene
  QN218_RS06275 - 1197605..1197784 (+) 180 WP_003236949.1 YqzE family protein -
  QN218_RS06280 - 1197826..1198152 (-) 327 WP_029316858.1 YqzG/YhdC family protein -
  QN218_RS06285 tapA 1198425..1199189 (+) 765 WP_060399014.1 amyloid fiber anchoring/assembly protein TapA -
  QN218_RS06290 - 1199173..1199745 (+) 573 WP_080429111.1 signal peptidase I -
  QN218_RS06295 tasA 1199809..1200594 (+) 786 WP_003236939.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39756.07 Da        Isoelectric Point: 9.4133

>NTDB_id=838548 QN218_RS06245 WP_060399019.1 1194654..1195691(+) (comGB) [Bacillus inaquosorum strain BSY82]
MKQIRKTWPLKDQAQLLKRLGEMTAGGYTLLDGLRLMELQMTKRQAADLTDTVIRLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQAIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILVALFAAGIGLYYWLVFKKKSPARQMLICVRIPLVGNLVRLFNSYFFSLQLSSLLKAGLSIYDSLNAFKHQT
FLPFYRCEAEQVIERLKAGESIEAAICESPFYETDFSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILIVYLSMLLPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=838548 QN218_RS06245 WP_060399019.1 1194654..1195691(+) (comGB) [Bacillus inaquosorum strain BSY82]
ATGAAACAGATTAGGAAAACTTGGCCGTTAAAGGATCAAGCCCAGTTATTGAAGCGGCTCGGTGAAATGACGGCGGGCGG
GTATACCCTTCTGGATGGATTACGCCTGATGGAACTCCAGATGACTAAAAGGCAGGCGGCTGACTTGACTGACACAGTCA
TTCGTTTGAGGGAAGGGGCGCCGTTTTATCAAGTATTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAAAGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGGGTGCTGCGATATCCGCTTTTCCTCATCTTCACAGTCGCTGTCATGTTTTATATGTTACAGGCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACAGACATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTCTGGTGGCTCTTTTTGCAGCAGGTATAGGCCTTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCTAGGCAAATGCTTATTTGTGTCAGGATTCCTTTGGTTGGAAACCTTGTCAGGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAAGCAGGCCTCTCAATTTATGACAGCCTTAACGCATTTAAACACCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAAGTGATTGAACGGTTAAAAGCTGGTGAATCAATTGAAGCCGCTATTTG
TGAAAGCCCTTTTTATGAAACTGATTTTTCAAAAGTCATATCTCACGGCCAGCTAAGCGGCCGCCTGGATCGGGAGCTTT
TCACATACAGCCAGTTCATACTTCAGCGGCTGGAACACAAGGCGCAAAAATGGACAGGCATCCTTCAGCCTATGATTTAT
GGATTTGTTGCAGCAATGATCTTAATCGTGTATTTATCTATGCTCTTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

93.808

93.623

0.878