Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   QPL77_RS05190 Genome accession   NZ_CP126521
Coordinates   1073965..1077669 (+) Length   1234 a.a.
NCBI ID   WP_211063201.1    Uniprot ID   -
Organism   Bacillus pumilus strain Monterrey_S2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1068965..1082669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL77_RS05175 (QPL77_05175) - 1069259..1069669 (+) 411 WP_144522812.1 DoxX family protein -
  QPL77_RS05180 (QPL77_05180) - 1069867..1070313 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  QPL77_RS05185 (QPL77_05185) addB 1070478..1073987 (+) 3510 WP_211063202.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QPL77_RS05190 (QPL77_05190) addA 1073965..1077669 (+) 3705 WP_211063201.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QPL77_RS05195 (QPL77_05195) sbcD 1077696..1078871 (+) 1176 WP_144522815.1 exonuclease subunit SbcD -
  QPL77_RS05200 (QPL77_05200) sbcC 1078868..1082263 (+) 3396 WP_211063200.1 exonuclease subunit SbcC -
  QPL77_RS05205 (QPL77_05205) - 1082283..1082582 (+) 300 WP_144522817.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142163.26 Da        Isoelectric Point: 5.2129

>NTDB_id=838300 QPL77_RS05190 WP_211063201.1 1073965..1077669(+) (addA) [Bacillus pumilus strain Monterrey_S2]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELANNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPKQWMRAFLSLYDVNTQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRVENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSSEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRGFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEKGEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLKQRDLLTDEQVQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYSGGIDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNVEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=838300 QPL77_RS05190 WP_211063201.1 1073965..1077669(+) (addA) [Bacillus pumilus strain Monterrey_S2]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
AGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACACC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAACGCATCTGCAGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAACAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTACGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGGAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACATCTGACCGTCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCAAGATCTCATCCATCTCCGAAGCAATGGATGAGGGCGTTCCTTTCACTTTATGATGTAAATACTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCCTATATCAAAGAAGATCTTTCGCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGTAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAGAAAGCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCGGTTGTGGAGACGCTTGTAGA
GCTAGTGATCCAATTTGGTGAGCGCTTCGAAAGAGCGAAGCAGGAAAAGTCTATTGTCGACTTTTCCGATTTAGAGCACT
ATTGCTTGCGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACAATTTTAAAACTTGTATCTAAAGGAGA
GCATTCTTCAGAGGGTAATCTGTTTATGGTTGGTGATGTCAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCGGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGT
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACGGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGGAACTTGCAGTATCGAGACAT
TGTCATTTTACTTCGCTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATCCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAGTTGGCACTGATTCGGACGAGTGA
TAAAAAAGGTACGTATTATGATGCTGTAAAGGCCTTTATGAGTGTGACTCATTCTGATCATCCTACATGCAAAAAACTAG
AACGATTTTTCCAATTACTGCGCAAATGGCGTGGTTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGCGATACACAGTATCTTGATTACGTTGGGGGAATGCCTGGAGGTAAGCAGCGGCAAGCGAATCTTCGTGCCCTTTATGA
CCGGGCCAAGCAGTACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTACGTTTTATTGAAAGAATGCAGGAACGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATTCATAGTAGTAAAGGA
TTAGAATTCCCAGTCGTCTTTACCGCAGGACTTGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAGCTAAGGGTTTTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCAGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGAAAGGCGAGATTGTACAAGCATTGATGGATGGTCTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCCATGCATACGGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAGTCACGTATAGGACACTTGCTCGAATCCTTAAAGCAGC
GAGACTTACTAACAGATGAGCAGGTTCAGTCAATTAATCAAGAGGGAATCGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACGGAGGGAGAGCCTGTTCTAATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTATTAG
ATTATAAAACTGATAGAGTTCAAGGCAGATATTCAGGTGGAATTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAA
ACCCAAATCGCGTTATATGCAAAAGCGGTAGAGCGTTTGACAAACAGAACTTTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATGTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.343

100

0.665