Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QPL77_RS05185 Genome accession   NZ_CP126521
Coordinates   1070478..1073987 (+) Length   1169 a.a.
NCBI ID   WP_211063202.1    Uniprot ID   -
Organism   Bacillus pumilus strain Monterrey_S2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1065478..1078987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPL77_RS05155 (QPL77_05155) lepB 1065988..1066500 (+) 513 WP_041815362.1 signal peptidase I -
  QPL77_RS05160 (QPL77_05160) - 1066536..1067681 (-) 1146 WP_144522837.1 AbrB family transcriptional regulator -
  QPL77_RS05165 (QPL77_05165) - 1067948..1068370 (+) 423 WP_003210872.1 BlaI/MecI/CopY family transcriptional regulator -
  QPL77_RS05170 (QPL77_05170) - 1068363..1069202 (+) 840 WP_144522811.1 M56 family metallopeptidase -
  QPL77_RS05175 (QPL77_05175) - 1069259..1069669 (+) 411 WP_144522812.1 DoxX family protein -
  QPL77_RS05180 (QPL77_05180) - 1069867..1070313 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  QPL77_RS05185 (QPL77_05185) addB 1070478..1073987 (+) 3510 WP_211063202.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QPL77_RS05190 (QPL77_05190) addA 1073965..1077669 (+) 3705 WP_211063201.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QPL77_RS05195 (QPL77_05195) sbcD 1077696..1078871 (+) 1176 WP_144522815.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134777.76 Da        Isoelectric Point: 5.1979

>NTDB_id=838299 QPL77_RS05185 WP_211063202.1 1070478..1073987(+) (addB) [Bacillus pumilus strain Monterrey_S2]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISVESMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLAQQIPAAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKVTAAFTVDQS
YHDRQPSELDLFRMTGKTYFQLYQLAKECGADISENILERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSASK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKKLKKAQKVREMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSPLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCITYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGIEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQIIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=838299 QPL77_RS05185 WP_211063202.1 1070478..1073987(+) (addB) [Bacillus pumilus strain Monterrey_S2]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGACTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTAGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCTGAGGATATTGAGAAAATTTCAGTGGAGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAAATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTACTTGCCCAGCAAATTCCTGCAGCTGAAGAAATAAAAGGAGCACATATTTATATCGATGGTTTTTATCAATTTA
CACCGCAACAGCTTCTTGTCATCGAACAGCTCTTGCTGCATGCAGCAAAAGTAACTGCGGCTTTTACAGTAGATCAATCC
TATCATGATAGGCAGCCAAGTGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCGAA
AGAGTGCGGAGCTGACATTTCTGAAAATATTCTTGAAAGAAATCATCGACACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTGCAACCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAAGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTTAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATCGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTAAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGCGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTACGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTAAAAGAATGG
ATCGCGCCTCCGCTTTACCAGTTGCAGAAAAAGCTCAAAAAAGCGCAAAAGGTAAGGGAGATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGACTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTCATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAAATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTAATTCCGCCATCACTTGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCTTCTGATGAGAGTGTATTATCTGAAGATGACCGAGAATGGTTAAAGCGAGCAGGAGCAGAGTTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTATATTT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGCCACTTATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCTTATACCGTCTCACAGCTCCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAATATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGATCATATTCAAGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGGCAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAATCTAACGAAGG
AAGATTGTATCACTTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAGGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGACCTTTACCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTAGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAGGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
TCTTACCATTACGTATTCGAAAGAGTGGCTCGGAATAGAAGCAACGCCTGCTGGTATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGCTTA
TTGCTTGAAGATGTGGAAGCAGTCAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCACAAATCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCTGCGGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGTGAAAGAATTACAAATGGAGAGGTCGAAATTAATCCATACAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.322

99.316

0.659