Detailed information NTG0835816

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QNH45_RS05810
• Genome accession: NZ_CP126099
• Coordinates: 1128458..1132183 (+)
• Length: 1241 a.a.
• NCBI ID: WP_283884580.1
• Uniprot ID: A0AA95LUR9
• Organism: Bacillus wiedmannii strain LN15
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1123458..1137183

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QNH45_RS05795 (QNH45_05795)	-	1123475..1124068 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
QNH45_RS05800 (QNH45_05800)	lepB	1124266..1124829 (+)	564	WP_000751908.1	signal peptidase I	-
QNH45_RS05805 (QNH45_05805)	addB	1124946..1128461 (+)	3516	WP_283884578.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QNH45_RS05810 (QNH45_05810)	addA	1128458..1132183 (+)	3726	WP_283884580.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QNH45_RS05815 (QNH45_05815)	-	1132196..1132483 (+)	288	WP_283884581.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
QNH45_RS05820 (QNH45_05820)	gerPF	1132564..1132779 (-)	216	WP_001141570.1	spore germination protein GerPF	-
QNH45_RS05825 (QNH45_05825)	-	1132821..1133207 (-)	387	WP_283884582.1	spore germination protein GerPE	-
QNH45_RS05830 (QNH45_05830)	gerPD	1133223..1133417 (-)	195	WP_001102342.1	spore germination protein GerPD	-
QNH45_RS05835 (QNH45_05835)	gerPC	1133424..1134038 (-)	615	WP_283884583.1	spore germination protein GerPC	-
QNH45_RS05840 (QNH45_05840)	gerPB	1134107..1134313 (-)	207	WP_048559586.1	spore germination protein GerPB	-
QNH45_RS05845 (QNH45_05845)	gerPA	1134328..1134549 (-)	222	WP_001111187.1	spore germination protein GerPA	-
QNH45_RS05850 (QNH45_05850)	-	1134643..1134822 (-)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
QNH45_RS05855 (QNH45_05855)	-	1135069..1135968 (+)	900	WP_283884584.1	fumarylacetoacetate hydrolase family protein	-
QNH45_RS05860 (QNH45_05860)	-	1136002..1136286 (-)	285	WP_283884585.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142584.75 Da
• Isoelectric Point: 4.8476

>NTDB_id=835816 QNH45_RS05810 WP_283884580.1 1128458..1132183(+) (addA) [Bacillus wiedmannii strain LN15]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPVPRVETLQADAVLLGTLSSAACESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTGRFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPDGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLQWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGKTLFAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEVAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=835816 QNH45_RS05810 WP_283884580.1 1128458..1132183(+) (addA) [Bacillus wiedmannii strain LN15]
ATGATTGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACAATCCATTCATTTTGTTTACAAGTAATTAGAGGATATTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGACCGTTATACGAGTGACCGTAGTGATGACGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCTTACCTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGTGCCCCGCGTTGAAACACTGCAAGCAGATGCAGTTTTACT
TGGAACGTTATCATCAGCAGCATGTGAATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGGGCGTTTCCAAGCGATGAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTCTACAAATTTTAAGTGAACAAAGTGAAAACGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGCGACGTAAAACAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTTCTAGGAAAGTATAAACGCTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATAGATTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTACGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGATGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGTTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCGCAACTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTAGACTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATTGATAAT
CCGATGCAAGATATTCCACTTGCCGCCGTACTTCGTTCACCAATCGTTGGACTAAATGATGAAGAACTTGCAACATTGCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAACAAGAAT
TACATGATAAATTACAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGGCAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGAACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTACTTGAATTAGGACAAGGAAGCATTCCGGGTGAAATTTATGGATATGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTATTTGCACCGGAACCGGTTCAAGAAGAAAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAACGGAAAGAAGAAGTGTATGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTGACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCACCAATTAAAACGCGTCCTCGTTTTATGGAGAAAAAAGGATTAACGTATGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGAGAGCAAATTGCCG
GAATGGTAAATAAGGAATTATTAACATTCGAACAAGCTGAAGAAGTAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAGAGGGTATTAGCAGCGAAAAGTGTTGAGCGTGAAGTACCATTTACAATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAAACGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGATTTTAAAACGGATACGATTGAAGGAAAGTTCCCAGGTGGATTCGATCAAGCAAAACCAATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTATATGCAAAAGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGTAATCATGTTGTTAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.087	100	0.533