Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QNH45_RS05805 Genome accession   NZ_CP126099
Coordinates   1124946..1128461 (+) Length   1171 a.a.
NCBI ID   WP_283884578.1    Uniprot ID   A0AA95LW47
Organism   Bacillus wiedmannii strain LN15     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1119946..1133461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH45_RS05770 (QNH45_05770) - 1119953..1120744 (-) 792 WP_283884573.1 phosphotransferase -
  QNH45_RS05775 (QNH45_05775) cspA 1121119..1121322 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  QNH45_RS05780 (QNH45_05780) - 1121514..1121714 (-) 201 WP_000412674.1 hypothetical protein -
  QNH45_RS05785 (QNH45_05785) - 1122035..1122220 (-) 186 WP_283884575.1 hypothetical protein -
  QNH45_RS05790 (QNH45_05790) - 1122504..1123085 (+) 582 WP_283884577.1 competence protein ComK -
  QNH45_RS05795 (QNH45_05795) - 1123475..1124068 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  QNH45_RS05800 (QNH45_05800) lepB 1124266..1124829 (+) 564 WP_000751908.1 signal peptidase I -
  QNH45_RS05805 (QNH45_05805) addB 1124946..1128461 (+) 3516 WP_283884578.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QNH45_RS05810 (QNH45_05810) addA 1128458..1132183 (+) 3726 WP_283884580.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QNH45_RS05815 (QNH45_05815) - 1132196..1132483 (+) 288 WP_283884581.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  QNH45_RS05820 (QNH45_05820) gerPF 1132564..1132779 (-) 216 WP_001141570.1 spore germination protein GerPF -
  QNH45_RS05825 (QNH45_05825) - 1132821..1133207 (-) 387 WP_283884582.1 spore germination protein GerPE -
  QNH45_RS05830 (QNH45_05830) gerPD 1133223..1133417 (-) 195 WP_001102342.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134325.49 Da        Isoelectric Point: 5.2121

>NTDB_id=835815 QNH45_RS05805 WP_283884578.1 1124946..1128461(+) (addB) [Bacillus wiedmannii strain LN15]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKEGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEILRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRVRAEESGDFLFATDHEQVWEEVMSLLDMFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGSGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=835815 QNH45_RS05805 WP_283884578.1 1124946..1128461(+) (addB) [Bacillus wiedmannii strain LN15]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
GCAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATCGGGA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTGCTTCGTAAAATTGTTGAGTCTCGTAAAGAGGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGACGCACATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGCTAGTCGAAAAGCTTCCACAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TTTCACCGCAAGAACTAGAAATTTTAAGACAGCTTATGATTTGCGGCGCGAGAGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGGGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCGGATGAAGATTATCGTTACCGAGATATTGCAGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCGCTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAT
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCGGTTTA
TTTATTTTTAGAAGAGCTGGACGTTCCGAAAAAACTAGAAGCATTACGTGTTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATATGTTCGTTGAGATGCTTGGTGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTGCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCCTTAAGAGAGCGTGATATTTTCAAATTGGATGC
GCCAGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTAGATTTAGAAGTGCCATTCGGTATGGGCGGTTCAGGATCACTTCCGCCGATGG
AATTTTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACAGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGAGGTACCGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAGGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGACTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGCAGCTTTAGTGCTCGTTCGAGTATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TATAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.319

100

0.494