Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ZMTM_RS08870 Genome accession   NZ_AP024110
Coordinates   1847816..1848997 (+) Length   393 a.a.
NCBI ID   WP_221763539.1    Uniprot ID   A0A8D5JRG5
Organism   Methyloradius palustris strain Zm11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1842816..1853997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZMTM_RS08850 (ZMTM_17060) - 1843274..1844014 (+) 741 WP_221763535.1 flagellar motor protein -
  ZMTM_RS08855 (ZMTM_17070) motD 1844029..1844940 (+) 912 WP_221763536.1 flagellar motor protein MotD -
  ZMTM_RS08860 (ZMTM_17080) - 1844955..1845995 (+) 1041 WP_221763537.1 Gfo/Idh/MocA family protein -
  ZMTM_RS08865 (ZMTM_17090) - 1846065..1847786 (+) 1722 WP_221763538.1 diguanylate cyclase -
  ZMTM_RS08870 (ZMTM_17100) pilU 1847816..1848997 (+) 1182 WP_221763539.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ZMTM_RS08875 (ZMTM_17110) - 1849080..1849538 (-) 459 WP_221763540.1 flagella synthesis protein FlgN -
  ZMTM_RS08880 (ZMTM_17120) flgM 1849560..1849868 (-) 309 WP_221763541.1 flagellar biosynthesis anti-sigma factor FlgM -
  ZMTM_RS08885 (ZMTM_17130) flgA 1850009..1850749 (-) 741 WP_221763542.1 flagellar basal body P-ring formation chaperone FlgA -
  ZMTM_RS08890 (ZMTM_17140) flgB 1850981..1851397 (+) 417 WP_221763543.1 flagellar basal body rod protein FlgB -
  ZMTM_RS08895 (ZMTM_17150) flgC 1851445..1851852 (+) 408 WP_221763544.1 flagellar basal body rod protein FlgC -
  ZMTM_RS08900 (ZMTM_17160) - 1851916..1852596 (+) 681 WP_221763545.1 flagellar hook assembly protein FlgD -
  ZMTM_RS08905 (ZMTM_17170) flgE 1852656..1853867 (+) 1212 WP_221763546.1 flagellar hook protein FlgE -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43986.30 Da        Isoelectric Point: 6.1556

>NTDB_id=83491 ZMTM_RS08870 WP_221763539.1 1847816..1848997(+) (pilU) [Methyloradius palustris strain Zm11]
MNNEQALAYMHTLLKSMLEKKASDLFISADFPPAMKIDGKMTPVTQQKLSGEHTQAFAHSLMNEKQRKEFEAEKECNFAI
WPREIGRFRVNVFVQQEKVGMVLRTITTKIPNFDEMGLPPVLKDVMMAKRGLIILVGGTGSGKSTTLAALIDYRNENSYG
HIITVEDPVEYVHPSKNCLITHREVGRDTNGWFAALKNTLRQAPDVILIGEIRDRETMEFALAFAETGHLCMATLHANSA
NQALDRIVNFFPEERHPQLHMDLSLNLKAFVSQRLVPKAGGGRCAAIEILLNSPLIGDLILKGETNMIKEVMAKSAELGM
QTFDQALFNLAEEGRITEDDALRNSDSINELRLKFKLHSKHSGTKDSSSRIEHLSLHDDDAKEEESTEETPAS

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=83491 ZMTM_RS08870 WP_221763539.1 1847816..1848997(+) (pilU) [Methyloradius palustris strain Zm11]
ATGAATAACGAACAAGCACTAGCGTATATGCATACCTTACTCAAATCGATGCTTGAGAAAAAAGCGTCTGATTTGTTTAT
CTCGGCAGATTTTCCACCTGCGATGAAAATCGACGGGAAAATGACGCCTGTCACGCAGCAAAAGCTTTCTGGAGAACACA
CCCAGGCTTTTGCGCATTCATTGATGAACGAAAAACAGCGCAAAGAGTTTGAAGCTGAAAAAGAATGTAACTTTGCTATC
TGGCCCAGAGAGATCGGTCGCTTCCGCGTTAATGTATTCGTGCAGCAAGAAAAAGTGGGCATGGTGTTGCGTACTATTAC
AACAAAAATCCCGAACTTTGATGAAATGGGTCTACCGCCTGTTTTAAAAGATGTAATGATGGCCAAACGTGGCCTCATTA
TTCTGGTAGGTGGTACTGGTTCAGGAAAATCAACCACGCTGGCAGCCTTGATCGATTATCGAAATGAAAATAGCTACGGT
CATATCATCACGGTTGAAGACCCCGTAGAGTATGTACACCCAAGCAAAAACTGCCTGATTACTCACCGGGAAGTTGGGCG
TGATACCAATGGCTGGTTTGCTGCACTCAAAAATACATTACGTCAGGCGCCTGATGTGATCCTGATTGGTGAAATTCGCG
ACCGTGAAACGATGGAGTTTGCCTTGGCCTTTGCTGAAACTGGTCACTTGTGTATGGCTACATTACACGCCAATAGTGCC
AATCAGGCATTGGATCGAATTGTTAACTTTTTCCCTGAAGAACGTCATCCACAATTGCACATGGACTTATCGCTGAATTT
AAAAGCATTTGTTTCACAACGGCTCGTACCTAAAGCGGGTGGTGGCAGATGCGCTGCTATTGAAATTTTGCTGAACTCGC
CCTTGATTGGCGATTTAATACTCAAAGGTGAGACCAACATGATTAAAGAAGTCATGGCTAAGTCAGCAGAACTAGGTATG
CAAACCTTTGATCAAGCCTTGTTTAACCTGGCTGAAGAAGGCCGCATTACTGAAGACGACGCATTGCGCAATTCGGATTC
AATTAATGAACTACGCCTGAAATTCAAGCTGCACAGCAAACATTCTGGCACTAAAGACTCATCGTCTCGTATTGAACATT
TATCGCTGCATGATGATGACGCTAAAGAAGAAGAATCCACAGAAGAAACACCTGCAAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.649

94.148

0.552
  pilU Acinetobacter baylyi ADP1

55.495

92.621

0.514
  pilU Vibrio cholerae strain A1552

53.161

88.55

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.619

84.224

0.384
  pilT Pseudomonas aeruginosa PAK

43.769

83.715

0.366
  pilT Acinetobacter baumannii D1279779

43.465

83.715

0.364
  pilT Acinetobacter baumannii strain A118

43.465

83.715

0.364
  pilT Acinetobacter nosocomialis M2

43.465

83.715

0.364

Multiple sequence alignment