Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QNH35_RS12185 Genome accession   NZ_CP126081
Coordinates   2368797..2369867 (-) Length   356 a.a.
NCBI ID   WP_044140966.1    Uniprot ID   A0AB34QT08
Organism   Bacillus pumilus strain B2_10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2363797..2374867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH35_RS12135 (QNH35_12135) - 2363890..2364462 (-) 573 WP_044140976.1 signal peptidase I -
  QNH35_RS12140 (QNH35_12140) tapA 2364514..2365041 (-) 528 WP_044140975.1 amyloid fiber anchoring/assembly protein TapA -
  QNH35_RS12145 (QNH35_12145) - 2365341..2365619 (+) 279 WP_283924529.1 DUF3889 domain-containing protein -
  QNH35_RS12150 (QNH35_12150) - 2365653..2365847 (-) 195 WP_106065585.1 YqzE family protein -
  QNH35_RS12155 (QNH35_12155) comGG 2365907..2366290 (-) 384 WP_283923821.1 competence type IV pilus minor pilin ComGG -
  QNH35_RS12160 (QNH35_12160) comGF 2366287..2366817 (-) 531 WP_283923822.1 competence type IV pilus minor pilin ComGF -
  QNH35_RS12165 (QNH35_12165) comGE 2366726..2367043 (-) 318 WP_283923823.1 competence type IV pilus minor pilin ComGE -
  QNH35_RS12170 (QNH35_12170) comGD 2367027..2367473 (-) 447 WP_283923824.1 competence type IV pilus minor pilin ComGD -
  QNH35_RS12175 (QNH35_12175) comGC 2367466..2367759 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  QNH35_RS12180 (QNH35_12180) comGB 2367776..2368855 (-) 1080 WP_260564470.1 competence type IV pilus assembly protein ComGB -
  QNH35_RS12185 (QNH35_12185) comGA 2368797..2369867 (-) 1071 WP_044140966.1 competence type IV pilus ATPase ComGA Machinery gene
  QNH35_RS12190 (QNH35_12190) - 2370046..2370918 (-) 873 WP_113767423.1 STAS domain-containing protein -
  QNH35_RS12200 (QNH35_12200) - 2371142..2371522 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QNH35_RS12205 (QNH35_12205) - 2371736..2371978 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  QNH35_RS12210 (QNH35_12210) - 2372024..2372662 (-) 639 WP_065098400.1 MBL fold metallo-hydrolase -
  QNH35_RS12215 (QNH35_12215) - 2372820..2372993 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  QNH35_RS12220 (QNH35_12220) - 2373216..2373530 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  QNH35_RS12225 (QNH35_12225) - 2373609..2374862 (-) 1254 WP_065098401.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40283.64 Da        Isoelectric Point: 9.0692

>NTDB_id=834765 QNH35_RS12185 WP_044140966.1 2368797..2369867(-) (comGA) [Bacillus pumilus strain B2_10]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=834765 QNH35_RS12185 WP_044140966.1 2368797..2369867(-) (comGA) [Bacillus pumilus strain B2_10]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATTGGAGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCCTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCCGAACGGCAATTAGAGCAGCACTAACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCATTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTTGGAAAGGAGCTCGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64