Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   Hhaem_RS04735 Genome accession   NZ_AP024093
Coordinates   948640..949182 (-) Length   180 a.a.
NCBI ID   WP_223892870.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain 2019-19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943640..954182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Hhaem_RS04705 (Hhaem_08930) fdx 944041..944382 (+) 342 WP_005645184.1 ISC system 2Fe-2S type ferredoxin -
  Hhaem_RS04710 (Hhaem_08940) iscX 944382..944576 (+) 195 WP_005645247.1 Fe-S cluster assembly protein IscX -
  Hhaem_RS04715 (Hhaem_08950) - 944627..945241 (-) 615 WP_118855823.1 YfgM family protein -
  Hhaem_RS04720 (Hhaem_08960) hisS 945259..946530 (-) 1272 WP_223894281.1 histidine--tRNA ligase -
  Hhaem_RS04725 (Hhaem_08970) ispG 946540..947646 (-) 1107 WP_005630108.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Hhaem_RS04730 (Hhaem_08980) - 947658..948569 (-) 912 WP_223892868.1 RodZ family helix-turn-helix domain-containing protein -
  Hhaem_RS04735 (Hhaem_08990) pilF/pilF2 948640..949182 (-) 543 WP_223892870.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Hhaem_RS04740 (Hhaem_09000) - 949283..950434 (-) 1152 WP_005630119.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  Hhaem_RS04745 (Hhaem_09010) - 950681..951559 (+) 879 WP_223892872.1 D-alanyl-D-alanine carboxypeptidase family protein -
  Hhaem_RS04750 (Hhaem_09020) - 951763..952644 (+) 882 WP_005626941.1 metal ABC transporter substrate-binding protein -
  Hhaem_RS04755 (Hhaem_09030) - 952644..953573 (+) 930 WP_223892874.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20909.48 Da        Isoelectric Point: 8.2514

>NTDB_id=83437 Hhaem_RS04735 WP_223892870.1 948640..949182(-) (pilF/pilF2) [Haemophilus haemolyticus strain 2019-19]
MQTISKQLNAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALGYLQQGNPQLAKINLDKALQHDKDYYLVHSALAHYYQ
RQGEIENAHREYEIALKLNNQQGDLHNNFGTFLCRQKQFEQAQQQFQLALNSPNYYHQADTFENMALCAYSSQKMDIYQQ
ALQKLRQTDSKRAEKFNTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=83437 Hhaem_RS04735 WP_223892870.1 948640..949182(-) (pilF/pilF2) [Haemophilus haemolyticus strain 2019-19]
ATGCAAACAATAAGTAAACAACTAAATGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCTCAATCAACAATTCA
AGATTTCAATCAACAATCAGCAGCGAAAGCTCGCGTAGAACTTGCCCTCGGTTACCTTCAACAAGGTAATCCTCAACTAG
CCAAAATCAACTTAGATAAAGCACTTCAACACGATAAAGATTACTATCTCGTGCATTCAGCGCTTGCGCATTATTATCAG
CGACAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCTTTAAAGCTCAATAATCAACAAGGAGACTTGCATAA
TAATTTTGGTACATTTTTATGTCGTCAAAAGCAATTTGAACAAGCTCAACAGCAATTTCAACTCGCACTGAATTCACCTA
ATTATTATCATCAAGCAGATACATTTGAAAATATGGCTCTTTGTGCTTATTCCTCTCAAAAAATGGATATTTATCAACAA
GCATTACAAAAATTGCGACAAACTGATAGTAAACGAGCGGAAAAATTTAATACCTTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

82.222

100

0.822
  pilF Glaesserella parasuis strain SC1401

46.703

100

0.472

Multiple sequence alignment