Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QMO40_RS04910 Genome accession   NZ_CP125805
Coordinates   987632..988189 (+) Length   185 a.a.
NCBI ID   WP_283386955.1    Uniprot ID   -
Organism   Mannheimia bovis strain 39324.S-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 982632..993189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMO40_RS04880 (QMO40_04880) - 982799..983608 (+) 810 WP_283386953.1 metal ABC transporter permease -
  QMO40_RS04885 (QMO40_04885) - 983656..984399 (-) 744 WP_025216715.1 metal ABC transporter ATP-binding protein -
  QMO40_RS04890 (QMO40_04890) - 984411..985307 (-) 897 WP_112110581.1 metal ABC transporter substrate-binding protein -
  QMO40_RS04895 (QMO40_04895) - 985556..986716 (+) 1161 WP_112110582.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QMO40_RS04900 (QMO40_04900) - 986759..987061 (-) 303 WP_283386954.1 helix-turn-helix transcriptional regulator -
  QMO40_RS04905 (QMO40_04905) - 987045..987416 (-) 372 WP_025235395.1 type II toxin-antitoxin system RelE/ParE family toxin -
  QMO40_RS04910 (QMO40_04910) pilF 987632..988189 (+) 558 WP_283386955.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QMO40_RS04915 (QMO40_04915) - 988198..990522 (+) 2325 WP_283387076.1 Tex family protein -
  QMO40_RS04920 (QMO40_04920) - 990563..991384 (-) 822 WP_283386956.1 SDR family NAD(P)-dependent oxidoreductase -
  QMO40_RS04925 (QMO40_04925) - 991386..991949 (-) 564 WP_283386957.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21174.03 Da        Isoelectric Point: 7.0817

>NTDB_id=832854 QMO40_RS04910 WP_283386955.1 987632..988189(+) (pilF) [Mannheimia bovis strain 39324.S-11]
MFAKFLKKITACMAIVLLAACSSQQESSSEPEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDIENAEKSYRTALNLSKNRPDVLNNYGTFLCKQGQFQQAYQQFEQAIESEVPYYHQADSLENIILCAKKEPNP
TRITETLTKLEKLDKPRATLLKESK

Nucleotide


Download         Length: 558 bp        

>NTDB_id=832854 QMO40_RS04910 WP_283386955.1 987632..988189(+) (pilF) [Mannheimia bovis strain 39324.S-11]
ATGTTTGCAAAATTTCTTAAAAAAATAACCGCTTGTATGGCGATAGTGTTATTGGCGGCGTGTTCTTCTCAGCAAGAATC
TTCTTCCGAACCTGAATTTAACCGCTCTGAAGCAGTGAAAGCCCGCATTAATCTTGCTTTAGCCTATTTGGAACAAAACG
ATTTCCCCAAAGCGAAACAGAATATTGATAAAGCCCTTGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTATTAGCC
TACTATTATCAACAAACAGGTGATATTGAAAATGCTGAAAAATCCTACCGAACAGCCTTAAATTTAAGTAAAAATCGCCC
TGATGTACTGAATAATTACGGCACTTTTTTGTGTAAACAGGGTCAATTTCAGCAAGCATATCAACAATTTGAGCAGGCAA
TCGAAAGTGAAGTGCCTTACTACCACCAAGCGGATAGCTTAGAAAACATCATCTTATGTGCGAAAAAAGAGCCAAATCCG
ACTAGAATAACCGAAACTTTAACGAAGCTCGAAAAATTAGATAAACCTCGAGCCACTTTATTGAAGGAAAGTAAATGA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(40-67)

Tetratricopeptide repeat (TPR_8)

(77-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

61.497

100

0.622
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.419

92.973

0.459