Detailed information NTG0832790

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMO65_RS02015
• Genome accession: NZ_CP125780
• Coordinates: 436298..437446 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPHA13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431298..442446

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMO65_RS01990 (QMO65_01990)	-	432449..432745 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QMO65_RS01995 (QMO65_01995)	-	432742..433335 (-)	594	WP_003084544.1	YggT family protein	-
QMO65_RS02000 (QMO65_02000)	proC	433346..434167 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QMO65_RS02005 (QMO65_02005)	-	434179..434871 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QMO65_RS02010 (QMO65_02010)	pilT	435086..436120 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QMO65_RS02015 (QMO65_02015)	pilU	436298..437446 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QMO65_RS02020 (QMO65_02020)	czcD	437454..438353 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QMO65_RS02025 (QMO65_02025)	-	438507..438911 (+)	405	WP_003084560.1	NINE protein	-
QMO65_RS02030 (QMO65_02030)	-	439154..440527 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QMO65_RS02035 (QMO65_02035)	-	440524..441708 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=832790 QMO65_RS02015 WP_003100959.1 436298..437446(+) (pilU) [Pseudomonas aeruginosa strain HPHA13]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=832790 QMO65_RS02015 WP_003100959.1 436298..437446(+) (pilU) [Pseudomonas aeruginosa strain HPHA13]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382