Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NSMS1_RS16825 Genome accession   NZ_AP023441
Coordinates   3886814..3888328 (-) Length   504 a.a.
NCBI ID   WP_224085768.1    Uniprot ID   -
Organism   Nostoc sp. MS1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3881814..3893328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSMS1_RS16795 (NSMS1_33080) - 3882783..3883583 (-) 801 WP_224085760.1 Uma2 family endonuclease -
  NSMS1_RS35340 (NSMS1_33090) - 3883656..3883832 (-) 177 WP_317986520.1 hypothetical protein -
  NSMS1_RS35345 - 3883935..3884027 (-) 93 WP_317986602.1 HNH endonuclease -
  NSMS1_RS16805 (NSMS1_33100) - 3884098..3884337 (-) 240 WP_224085763.1 hypothetical protein -
  NSMS1_RS16810 (NSMS1_33110) - 3884666..3885139 (+) 474 WP_224085765.1 hypothetical protein -
  NSMS1_RS16815 - 3885427..3885666 (-) 240 WP_224095258.1 WD40 repeat domain-containing protein -
  NSMS1_RS16820 (NSMS1_33120) - 3885972..3886727 (-) 756 WP_224085766.1 Uma2 family endonuclease -
  NSMS1_RS16825 (NSMS1_33130) radA 3886814..3888328 (-) 1515 WP_224085768.1 DNA repair protein RadA Machinery gene
  NSMS1_RS16830 (NSMS1_33140) rpaB 3888504..3889232 (+) 729 WP_067774651.1 response regulator transcription factor RpaB -
  NSMS1_RS16835 (NSMS1_33150) - 3889281..3889934 (+) 654 WP_224085770.1 cofactor assembly of complex C subunit B -
  NSMS1_RS16840 (NSMS1_33160) - 3889994..3890644 (+) 651 WP_224085772.1 Uma2 family endonuclease -
  NSMS1_RS16845 (NSMS1_33170) - 3890669..3891187 (+) 519 WP_224085775.1 DUF456 domain-containing protein -
  NSMS1_RS16850 (NSMS1_33180) - 3891188..3892057 (+) 870 WP_224085777.1 Rpn family recombination-promoting nuclease/putative transposase -
  NSMS1_RS16855 (NSMS1_33190) - 3892057..3892311 (+) 255 WP_224085779.1 DUF4351 domain-containing protein -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 54238.07 Da        Isoelectric Point: 4.8227

>NTDB_id=83254 NSMS1_RS16825 WP_224085768.1 3886814..3888328(-) (radA) [Nostoc sp. MS1]
MPKPKTFYICNECGAESPQWFGKCPACGTYNSLEEQLTIQSSVDVPKGGVSGWHAAQNNGKAVKPAKPRASLTFDQITDR
QIARWESGYGELDRVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSNQLAQRYRILYVTGEESGQQVKLRASRLGVAKTLNV
VGEDGEESEQTPQLSIAEGVGADLYVLPETDLEEILREVDSLKPNVAVIDSIQTVFFPALTSAPGSVAQVRECTAALMKV
AKHEDITMLIVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFASHRLLRTVKNRFGATHEIGIFEMVQNGLREVSNPSELF
IGNRDDPAPGTALVVACEGTRPIVVELQALVSPTSYPSPRRAGTGIDYNRLVQILAVLEKRVGIPMSKLDSYVASAGGLN
VEEPAVDLGIAIAIVASFRDRIVDPGTVLIGEVGLGGQVRSVSQMELRLKEAAKLGFKRAIVPKGQKFPDFDIEILPVAK
VIDAIIAAIPHQELTEDDLDMDEE

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=83254 NSMS1_RS16825 WP_224085768.1 3886814..3888328(-) (radA) [Nostoc sp. MS1]
ATGCCAAAGCCAAAAACCTTTTACATTTGTAACGAATGTGGTGCTGAATCTCCCCAATGGTTTGGTAAGTGTCCAGCTTG
TGGTACTTACAACTCCCTAGAAGAACAACTTACCATTCAATCTTCCGTAGATGTACCTAAAGGAGGTGTCAGTGGTTGGC
ACGCAGCCCAAAATAATGGAAAAGCAGTCAAGCCAGCAAAACCGCGCGCTTCATTAACATTTGACCAAATAACCGATCGC
CAAATTGCCCGTTGGGAATCTGGCTATGGAGAACTCGATCGGGTGCTTGGCGGTGGCGTAGTTCCGGGTTCTATGGTGTT
AATTGGCGGTGATCCTGGTATTGGTAAATCTACCTTATTATTACAAGTCTCAAACCAATTAGCGCAGAGATATCGTATCC
TTTATGTAACTGGTGAAGAATCAGGACAGCAAGTAAAATTAAGAGCTTCTCGCTTAGGTGTAGCAAAAACCTTAAACGTT
GTCGGAGAAGATGGTGAGGAGTCAGAACAAACACCTCAATTATCAATAGCTGAAGGTGTAGGTGCTGATTTATACGTCTT
ACCAGAAACAGATTTAGAAGAAATTTTACGAGAAGTCGATTCACTTAAACCCAATGTGGCAGTAATCGACAGTATTCAAA
CCGTATTTTTCCCTGCCCTTACCTCTGCACCCGGTTCAGTTGCTCAAGTAAGGGAATGTACAGCCGCCTTGATGAAGGTG
GCGAAACACGAAGACATCACCATGTTGATTGTTGGACACGTCACCAAGGAAGGGGCGATCGCCGGGCCGAAAGTTTTAGA
ACACTTAGTAGATACAGTACTGTATTTTGAAGGCGATCGCTTCGCCTCCCATCGGTTATTACGTACAGTCAAAAACCGCT
TCGGTGCAACTCACGAAATCGGTATTTTTGAAATGGTGCAGAACGGACTCCGTGAAGTCTCTAACCCCAGCGAGTTATTT
ATAGGCAACCGTGATGATCCTGCACCGGGTACTGCGCTCGTTGTCGCCTGCGAAGGTACACGCCCCATTGTAGTTGAGTT
ACAAGCCTTAGTCAGCCCTACCAGTTACCCATCCCCCAGACGGGCGGGTACTGGTATAGATTACAATCGCCTAGTGCAGA
TACTCGCCGTCTTAGAAAAGCGCGTCGGGATACCCATGTCGAAACTAGACTCCTACGTCGCCTCGGCTGGGGGGTTAAAT
GTAGAAGAACCAGCCGTCGATTTAGGTATCGCCATTGCCATTGTCGCCAGTTTCCGCGATCGCATCGTTGACCCAGGTAC
AGTATTGATAGGCGAAGTCGGCTTAGGTGGACAGGTGCGATCAGTTTCCCAAATGGAACTACGCCTCAAAGAAGCAGCTA
AACTAGGATTTAAACGTGCGATCGTTCCCAAGGGTCAAAAATTTCCCGACTTTGACATCGAAATTTTACCAGTTGCCAAA
GTTATAGATGCAATTATTGCCGCTATCCCCCATCAAGAATTGACAGAAGACGACTTAGACATGGACGAAGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(399-464)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

KaiC (ATPase)

(88-293)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50

97.222

0.486
  radA Streptococcus mitis SK321

45.455

96.032

0.437
  radA Streptococcus pneumoniae Rx1

46.767

92.063

0.431
  radA Streptococcus pneumoniae D39

46.767

92.063

0.431
  radA Streptococcus pneumoniae R6

46.767

92.063

0.431
  radA Streptococcus pneumoniae TIGR4

46.767

92.063

0.431
  radA Streptococcus mitis NCTC 12261

46.767

92.063

0.431

Multiple sequence alignment