Detailed information NTG0831026

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMB36_RS12740
• Genome accession: NZ_CP125300
• Coordinates: 3018056..3019225 (-)
• Length: 389 a.a.
• NCBI ID: WP_003400837.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae strain B48
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3013056..3024225

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMB36_RS12725 (QMB36_12725)	-	3014342..3015406 (+)	1065	WP_003400842.1	microcin C ABC transporter permease YejB	-
QMB36_RS12730 (QMB36_12730)	-	3015406..3016428 (+)	1023	WP_003400841.1	ABC transporter permease	-
QMB36_RS12735 (QMB36_12735)	-	3016430..3018007 (+)	1578	WP_024641463.1	ABC transporter ATP-binding protein	-
QMB36_RS12740 (QMB36_12740)	pilU	3018056..3019225 (-)	1170	WP_003400837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QMB36_RS12745 (QMB36_12745)	-	3019349..3019624 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
QMB36_RS12750 (QMB36_12750)	pgm	3019699..3021345 (-)	1647	WP_011268077.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QMB36_RS12755 (QMB36_12755)	-	3021509..3023836 (-)	2328	WP_054092646.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42577.65 Da
• Isoelectric Point: 6.0641

>NTDB_id=831026 QMB36_RS12740 WP_003400837.1 3018056..3019225(-) (pilU) [Pseudomonas syringae pv. syringae strain B48]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=831026 QMB36_RS12740 WP_003400837.1 3018056..3019225(-) (pilU) [Pseudomonas syringae pv. syringae strain B48]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TGATGGACGAAGAACAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAACTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACGGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATCGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
GCTGGTCAGAACCCCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCTGCGCTA
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCAGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362