Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   QMA14_RS10345 Genome accession   NZ_CP125087
Coordinates   2136963..2137505 (-) Length   180 a.a.
NCBI ID   WP_284173925.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain EL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2131963..2142505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMA14_RS10320 (QMA14_10320) - 2132323..2132766 (-) 444 WP_284173922.1 isoprenylcysteine carboxylmethyltransferase family protein -
  QMA14_RS10325 (QMA14_10325) - 2132818..2133432 (-) 615 WP_049367064.1 YfgM family protein -
  QMA14_RS10330 (QMA14_10330) hisS 2133451..2134722 (-) 1272 WP_197555852.1 histidine--tRNA ligase -
  QMA14_RS10335 (QMA14_10335) ispG 2134732..2135838 (-) 1107 WP_005695590.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QMA14_RS10340 (QMA14_10340) - 2135857..2136888 (-) 1032 WP_284173924.1 RodZ family helix-turn-helix domain-containing protein -
  QMA14_RS10345 (QMA14_10345) pilF/pilF2 2136963..2137505 (-) 543 WP_284173925.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QMA14_RS10350 (QMA14_10350) - 2137575..2138723 (-) 1149 WP_197542296.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QMA14_RS10355 (QMA14_10355) - 2139025..2139900 (+) 876 WP_284173926.1 D-alanyl-D-alanine carboxypeptidase family protein -
  QMA14_RS10360 (QMA14_10360) - 2140055..2140936 (+) 882 WP_284173927.1 metal ABC transporter substrate-binding protein -
  QMA14_RS10365 (QMA14_10365) - 2140936..2141832 (+) 897 WP_005695582.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20344.92 Da        Isoelectric Point: 5.9862

>NTDB_id=829906 QMA14_RS10345 WP_284173925.1 2136963..2137505(-) (pilF/pilF2) [Haemophilus parainfluenzae strain EL1]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQEDFVQAKLNLDKAFEHDDHYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENIALCAFAGKQTDVYQQ
ALDKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=829906 QMA14_RS10345 WP_284173925.1 2136963..2137505(-) (pilF/pilF2) [Haemophilus parainfluenzae strain EL1]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTTTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCAGCAAAAGCCCGAGTTGAATTAGCATTAGGCTATCTGCAACAAGAGGATTTTGTTCAAG
CAAAACTGAATTTAGATAAAGCCTTTGAACATGATGACCACTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGTTAGATGATAAGCAAGGCGATGTGTATAA
CAACTTTGGTGCATTTCTCTGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCGGCCCTCGCTGCACCAA
ATTATTATCATCAAGCGGATACTTACGAAAACATAGCGCTTTGTGCTTTTGCTGGAAAACAAACCGATGTTTATCAACAG
GCATTGGATAAATTGCGTCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

65.698

95.556

0.628
  pilF Glaesserella parasuis strain SC1401

49.718

98.333

0.489