Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QJS65_RS12505 Genome accession   NZ_CP124831
Coordinates   2400376..2401446 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus altitudinis strain Sample7_7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395376..2406446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJS65_RS12455 (QJS65_12455) - 2395471..2396043 (-) 573 WP_017357979.1 signal peptidase I -
  QJS65_RS12460 (QJS65_12460) tapA 2396093..2396620 (-) 528 WP_045034369.1 amyloid fiber anchoring/assembly protein TapA -
  QJS65_RS12465 (QJS65_12465) - 2396891..2397199 (+) 309 WP_282747444.1 DUF3889 domain-containing protein -
  QJS65_RS12470 (QJS65_12470) - 2397234..2397428 (-) 195 WP_024718846.1 YqzE family protein -
  QJS65_RS12475 (QJS65_12475) comGG 2397489..2397872 (-) 384 WP_282745260.1 competence type IV pilus minor pilin ComGG -
  QJS65_RS12480 (QJS65_12480) comGF 2397869..2398396 (-) 528 WP_218646143.1 competence type IV pilus minor pilin ComGF -
  QJS65_RS12485 (QJS65_12485) comGE 2398308..2398622 (-) 315 WP_007501227.1 competence type IV pilus minor pilin ComGE -
  QJS65_RS12490 (QJS65_12490) comGD 2398606..2399052 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  QJS65_RS12495 (QJS65_12495) comGC 2399045..2399338 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  QJS65_RS12500 (QJS65_12500) comGB 2399355..2400395 (-) 1041 WP_282745261.1 competence type IV pilus assembly protein ComGB -
  QJS65_RS12505 (QJS65_12505) comGA 2400376..2401446 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  QJS65_RS12510 (QJS65_12510) - 2401904..2402776 (-) 873 WP_041506953.1 STAS domain-containing protein -
  QJS65_RS12520 (QJS65_12520) - 2403000..2403380 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  QJS65_RS12525 (QJS65_12525) - 2403593..2403835 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  QJS65_RS12530 (QJS65_12530) - 2403946..2405301 (-) 1356 WP_282745230.1 IS1182 family transposase -
  QJS65_RS12535 (QJS65_12535) - 2405413..2406051 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  QJS65_RS12540 (QJS65_12540) - 2406212..2406385 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=828219 QJS65_RS12505 WP_024718839.1 2400376..2401446(-) (comGA) [Bacillus altitudinis strain Sample7_7]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=828219 QJS65_RS12505 WP_024718839.1 2400376..2401446(-) (comGA) [Bacillus altitudinis strain Sample7_7]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTCAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTCG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACAATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649