Detailed information NTG0828059

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QLG20_RS22220
• Genome accession: NZ_CP124804
• Coordinates: 4540119..4540919 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain J57
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4535119..4545919

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QLG20_RS22185 (QLG20_22175)	gspH	4535272..4535787 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
QLG20_RS22190 (QLG20_22180)	gspI	4535784..4536149 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
QLG20_RS22195 (QLG20_22185)	gspJ	4536146..4536742 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
QLG20_RS22200 (QLG20_22190)	gspK	4536739..4537719 (+)	981	WP_023297078.1	type II secretion system minor pseudopilin GspK	-
QLG20_RS22205 (QLG20_22195)	gspL	4537712..4538908 (+)	1197	WP_023339600.1	type II secretion system protein GspL	-
QLG20_RS22210 (QLG20_22200)	-	4538901..4539386 (+)	486	WP_124037163.1	type II secretion system protein M	-
QLG20_RS22215 (QLG20_22205)	-	4539376..4540122 (+)	747	WP_064142873.1	type II secretion system protein N	-
QLG20_RS22220 (QLG20_22210)	pilD	4540119..4540919 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
QLG20_RS22225 (QLG20_22215)	hrpB	4540942..4543371 (-)	2430	WP_124037165.1	ATP-dependent helicase HrpB	-
QLG20_RS22230 (QLG20_22220)	thpR	4543444..4543980 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
QLG20_RS22235 (QLG20_22225)	sfsA	4543980..4544696 (+)	717	WP_124037167.1	DNA/RNA nuclease SfsA	-
QLG20_RS22240 (QLG20_22230)	dksA	4544859..4545314 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=828059 QLG20_RS22220 WP_008807384.1 4540119..4540919(+) (pilD) [Klebsiella variicola strain J57]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=828059 QLG20_RS22220 WP_008807384.1 4540119..4540919(+) (pilD) [Klebsiella variicola strain J57]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425