Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QF117_RS06950 Genome accession   NZ_CP124614
Coordinates   221058..222056 (+) Length   332 a.a.
NCBI ID   WP_282388352.1    Uniprot ID   -
Organism   Vibrio sp. YMD68     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 216058..227056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QF117_RS06935 (QF117_06935) - 216450..217178 (-) 729 WP_282388349.1 glutathione peroxidase -
  QF117_RS06940 (QF117_06940) oxyR 217368..218255 (+) 888 WP_282388350.1 DNA-binding transcriptional regulator OxyR -
  QF117_RS06945 (QF117_06945) - 218326..220917 (-) 2592 WP_282388351.1 PBP1A family penicillin-binding protein -
  QF117_RS06950 (QF117_06950) pilM 221058..222056 (+) 999 WP_282388352.1 type IV pilus assembly protein PilM Machinery gene
  QF117_RS06955 (QF117_06955) - 222046..222582 (+) 537 Protein_194 PilN domain-containing protein -
  QF117_RS06960 (QF117_06960) pilO 222749..223339 (+) 591 WP_282388353.1 type 4a pilus biogenesis protein PilO Machinery gene
  QF117_RS06965 (QF117_06965) pilP 223329..223844 (+) 516 WP_282388354.1 pilus assembly protein PilP Machinery gene
  QF117_RS06970 (QF117_06970) - 223961..225580 (+) 1620 Protein_197 type IV pilus secretin PilQ family protein -
  QF117_RS06975 (QF117_06975) aroK 225769..226287 (+) 519 WP_017036110.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37385.91 Da        Isoelectric Point: 7.0577

>NTDB_id=826762 QF117_RS06950 WP_282388352.1 221058..222056(+) (pilM) [Vibrio sp. YMD68]
MGKPLITGIDIGHYSIKAVVLKPASDDYSLVSFKELPISGDVFSDNHTVNHQQIVNKLKELRKFLPIWSKRVAIAIPDNA
VISKVLQIDSDLDERETEFAIYQGFSHQSPFPIEELSLDFVRVDNDSKTQTVAYQVYATKKDVVDSRYQSIKKAGFLPIV
CDVQAHGLVSVWRQAAKMTANTDWLLVDVGYTQTSLCMDFDNKAHFSKEMAIGTQYLEQSSSPMNALLEKRIDQFIQLMI
DRIQRQIQMLLSVNGNSAVKGIWLSGGGATTPMLVEEIQRRLCIECQVLNPLSLLGRSRTRKPHHYSDLHGFNTAVGIAM
RGHDWLEVQHAL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=826762 QF117_RS06950 WP_282388352.1 221058..222056(+) (pilM) [Vibrio sp. YMD68]
ATGGGTAAACCATTAATAACTGGTATAGATATTGGCCACTATAGTATTAAAGCCGTGGTCTTAAAGCCAGCAAGTGATGA
CTATTCACTGGTAAGTTTTAAAGAATTGCCCATTTCAGGTGATGTATTCTCTGATAATCACACCGTAAATCATCAACAAA
TTGTCAATAAGTTAAAAGAGCTACGAAAATTTCTCCCTATTTGGAGTAAAAGGGTCGCGATTGCTATTCCTGATAACGCG
GTGATCAGCAAAGTACTGCAAATTGACAGCGATCTTGACGAGCGTGAGACAGAGTTTGCCATCTATCAAGGCTTCTCCCA
TCAGTCTCCCTTCCCCATTGAAGAACTCAGTTTGGACTTTGTGCGGGTTGATAACGACAGCAAAACGCAGACCGTTGCCT
ATCAAGTTTATGCCACCAAAAAAGACGTGGTTGACAGTCGTTATCAATCCATCAAAAAAGCCGGGTTTTTGCCGATCGTG
TGTGACGTTCAAGCTCATGGGTTAGTGAGCGTTTGGCGGCAGGCAGCAAAAATGACCGCCAACACAGATTGGTTGTTGGT
GGATGTTGGATACACCCAAACCTCTCTGTGTATGGACTTTGATAATAAGGCTCATTTTAGTAAAGAGATGGCTATTGGGA
CGCAATACCTAGAGCAATCTTCATCACCGATGAATGCGTTACTTGAGAAGAGAATCGATCAATTTATTCAGTTGATGATT
GACCGAATTCAACGGCAAATACAGATGTTGCTGTCCGTGAACGGCAATTCAGCAGTGAAAGGAATTTGGCTGTCTGGGGG
CGGCGCGACAACGCCAATGTTGGTTGAAGAGATTCAGCGTCGGCTTTGTATTGAATGCCAGGTTCTAAATCCTCTGTCTT
TATTGGGGCGCTCACGGACTAGAAAGCCACATCATTACAGTGATTTGCATGGTTTTAATACCGCCGTGGGGATCGCAATG
AGAGGTCACGATTGGCTGGAGGTTCAACATGCTCTATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.464

100

0.551
  pilM Vibrio campbellii strain DS40M4

43.03

99.398

0.428