Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QA541_RS06510 Genome accession   NZ_CP124577
Coordinates   1248808..1249779 (-) Length   323 a.a.
NCBI ID   WP_219492120.1    Uniprot ID   -
Organism   Macrococcus psychrotolerans strain NRL/St 21/332     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1243808..1254779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QA541_RS06475 (QA541_06470) gcvT 1244505..1245590 (-) 1086 WP_219494831.1 glycine cleavage system aminomethyltransferase GcvT -
  QA541_RS06480 (QA541_06475) - 1245728..1246234 (-) 507 WP_420493773.1 shikimate kinase -
  QA541_RS06485 (QA541_06480) comGF 1246395..1246847 (-) 453 WP_420493774.1 competence type IV pilus minor pilin ComGF -
  QA541_RS06490 (QA541_06485) - 1246792..1247034 (-) 243 WP_219491941.1 hypothetical protein -
  QA541_RS06495 (QA541_06490) comGD 1247021..1247446 (-) 426 WP_219491944.1 competence type IV pilus minor pilin ComGD -
  QA541_RS06500 (QA541_06495) comGC 1247439..1247762 (-) 324 WP_283722000.1 competence type IV pilus major pilin ComGC Machinery gene
  QA541_RS06505 (QA541_06500) comGB 1247784..1248857 (-) 1074 WP_283721886.1 competence type IV pilus assembly protein ComGB -
  QA541_RS06510 (QA541_06505) comGA 1248808..1249779 (-) 972 WP_219492120.1 competence type IV pilus ATPase ComGA Machinery gene
  QA541_RS06515 (QA541_06510) - 1249991..1250239 (+) 249 WP_041636023.1 DUF2626 domain-containing protein -
  QA541_RS06520 (QA541_06515) - 1250267..1250887 (-) 621 WP_219492123.1 MBL fold metallo-hydrolase -
  QA541_RS06525 (QA541_06520) - 1250977..1251216 (+) 240 WP_219492126.1 DUF2759 domain-containing protein -
  QA541_RS06530 (QA541_06525) - 1251243..1251554 (-) 312 WP_086038848.1 MTH1187 family thiamine-binding protein -
  QA541_RS06535 (QA541_06530) - 1251554..1252522 (-) 969 WP_413474511.1 ROK family glucokinase -
  QA541_RS06540 (QA541_06535) - 1252519..1252710 (-) 192 WP_133445012.1 YqgQ family protein -
  QA541_RS06545 (QA541_06540) - 1252715..1254163 (-) 1449 WP_420493775.1 rhomboid family intramembrane serine protease -
  QA541_RS06550 (QA541_06545) - 1254139..1254693 (-) 555 WP_211517715.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37050.64 Da        Isoelectric Point: 6.4160

>NTDB_id=826462 QA541_RS06510 WP_219492120.1 1248808..1249779(-) (comGA) [Macrococcus psychrotolerans strain NRL/St 21/332]
MERLFNEIIEQAILQSASDIHFIPGDKSISIKFRIQGDIEDYSDIDDILFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
NNLRYNIRASTLPRSLGDEACVLRIIRQSFIDEYQTDDQILFNQMKKSSGIIIISGPTGSGKSTLMYQLVHFAKNTLKRQ
VISIEDPVEQHIDGIIQVNVNEKAEITYQTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLVKRIDGKERVLAYEFLTKKDIEKYLKDKHINYRTLADIFKEMYETNQISQHEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=826462 QA541_RS06510 WP_219492120.1 1248808..1249779(-) (comGA) [Macrococcus psychrotolerans strain NRL/St 21/332]
ATGGAGAGATTATTCAATGAAATAATAGAGCAGGCAATTTTACAAAGTGCATCAGATATACACTTCATTCCTGGTGATAA
AAGCATATCTATTAAATTTAGGATACAAGGTGATATTGAAGACTATAGCGACATCGACGATATATTATTTAGAAAACTAC
TTTCATATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCCCAGAGTGGCATAATACATTTTAACCTG
AATAACTTACGATACAATATTCGCGCATCTACTTTACCTCGGTCATTAGGTGATGAAGCATGTGTATTGAGGATTATCAG
ACAAAGTTTTATAGATGAATATCAGACAGATGATCAAATATTGTTCAATCAAATGAAAAAATCAAGCGGTATAATTATCA
TTAGTGGACCAACTGGAAGTGGTAAAAGTACATTAATGTATCAACTTGTACATTTTGCGAAGAATACATTGAAACGACAA
GTAATTTCAATAGAAGATCCTGTAGAACAGCATATTGACGGTATCATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
GTATCAAACCGCGATAAAGGCAATCCTTAGATGTGATCCAGATATCATTATGCTAGGTGAAATAAGAGATTCAGTCGTAG
CCCAGCAAGTTATCAATGCAGGGTTAAGCGGGCATTTAGTTTTAACGACATTACATGCGAATGATTGCATCGGCGCATTA
TTTCGACTTAAAGAAATGGGAATTAATGCTGTTGATCTATATCAAAGTATTAATTTAATTATCAATCAAAGACTTGTAAA
AAGAATAGATGGAAAGGAGCGTGTTTTAGCCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTAAAGGATAAAC
ATATAAACTATAGAACGCTGGCTGACATATTTAAGGAGATGTATGAAACAAATCAGATTTCACAACATGAATTTGAAAAA
TTTGATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

49.695

100

0.505
  comGA Staphylococcus aureus N315

49.695

100

0.505
  comGA Bacillus subtilis subsp. subtilis str. 168

34.483

100

0.372